LOCUS       sequence-136890-        2208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent reporter of Orai1-associated calcium influx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: Orai1-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      299..308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      949..958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             970..1026
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     1239..1260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1366..1387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1627..1646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgcatccgg agcccgcccc gcccccgagc cgcagcagtc ccgagcttcc cccaagcggc
       61 ggcagcacca ccagcggcag ccgccggagc cgccgccgca gcggggacgg ggagcccccg
      121 ggggccccgc caccgccgcc gtccgccgtc acctacccgg actggatcgg ccagagttac
      181 tccgaggtga tgagcctcaa cgagcactcc atgcaggcgc tgtcctggcg caagctctac
      241 ttgagccgcg ccaagcttaa agcctccagc cggacctcgg ctctgctctc cggcttcgcc
      301 atggtggcaa tggtggaggt gcagctggac gctgaccacg actacccacc ggggctgctc
      361 atcgccttca gtgcctgcac cacagtgctg gtggctgtgc acctgtttgc gctcatgatc
      421 agcacctgca tcctgcccaa catcgaggcg gtgagcaacg tgcacaatct caactcggtc
      481 aaggagtccc cccatgagcg catgcaccgc cacatcgagc tggcctgggc cttctccacc
      541 gtcatcggca cgctgctctt cctagctgag gtggtgctgc tctgctgggt caagttcttg
      601 cccctcaaga agcagccagg ccagccaagg cccaccagca agccccccgc cagtggcgca
      661 gcagccaacg tcagcaccag cggcatcacc ccgggccagg cagctgccat cgcctcgacc
      721 accatcatgg tgcccttcgg cctgatcttt atcgtcttcg ccgtccactt ctaccgctca
      781 ctggttagcc ataagaccga ccgacagttc caggagctca acgagctggc ggagtttgcc
      841 cgcttacagg accagctgga ccacagaggg gaccaccccc tgacgcccgg cagccactat
      901 gcccgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtc
      961 gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg
     1021 agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
     1081 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     1141 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
     1201 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1261 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
     1321 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1381 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
     1441 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1501 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1561 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     1621 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1681 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1741 aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga atttaaagag
     1801 gaattctccc tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg
     1861 gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat gatcaatgaa
     1921 gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga
     1981 aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt gtttgataag
     2041 gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag
     2101 aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
     2161 caggtaaact acgaagagtt tgtacaaatg atgacagcga agctttaa
//
