LOCUS       sequence-136897-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRuby2_NLSPUFa in pCR8 gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1404-pCR8-mRuby2_NLSPUFa
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             131..148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             152..184
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(188..196,197..211)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             218..928
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctccgaattc accggtgccg ccaccatggt
      121 gcggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      181 gggtcgggat ctgtacgacg atgacgataa ggatccgatg gtgtctaagg gcgaagagct
      241 gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt
      301 caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca tgaggatcaa
      361 agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcatgta
      421 tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta aacagtcctt
      481 tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt
      541 catgcaggac accagccttg aggatggctg tctcgtttac cacgtccaag tcagaggggt
      601 aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg agcctaatac
      661 agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg cactgaaagt
      721 tgatggtggt ggccatctgt cttgctcttt cgtaacaact tacaggtcaa aaaagaccgt
      781 cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa ggttagagga
      841 aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt tcgccgggct
      901 tggtggtggg atggacgagc tgtacaaggg aggcggcggt a
//
