LOCUS       sequence-136899-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFb pCR8 gateway donor vector for insertion of N-terminal domain
            (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in
            dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1406-pCR8-4xNLS_PUFb_2xNLS
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             142..162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             166..186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             190..210
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             301..321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tccgaattca ccggtgccgc caccatgatc
      121 gatggcggag gcggatctga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga
      181 aaggtagatc caaaaaagaa gagaaaggta ggatctaccg gatctagaaa cgatggtggt
      241 ggtggaagcg ggggtggggg cagcggtgga gggggaagcg ggcgcgccgg gatcctcccc
      301 cccaagaaaa agaggaaggt atctagaggc cgcagccgcc ttttggaaga ttttcgaaac
      361 aaccggtacc ccaatttaca actgcgggag attgctggac atataatgga attttcccaa
      421 gaccagcatg ggtccagatt cattcagctg aaactggagc gtgccacacc agctgagcgc
      481 cagcttgtct tcaatgaaat cctccaggct gcctaccaac tcatggtgga tgtgtttggt
      541 aattacgtca ttcagaagtt ctttgaattt ggcagtcttg aacagaagct ggctttggca
      601 gaacggattc gaggccacgt cctgtcattg gcactacaga tgtatggctg ccgtgttatc
      661 cagaaagctc ttgagtttat tccttcagac cagcagaatg agatggttcg ggaactagat
      721 ggccatgtct tgaagtgtgt gaaagatcag aatggcaatc acgtggttca gaaatgcatt
      781 gaatgtgtac agccccagtc tttgcaattt atcatcgacg cgtttaaggg acaggtattt
      841 gccttatcca cacatcctta tggctgccga gtgat
//
