LOCUS       sequence-136900-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCBPHAT_4xNLS_PUFa_2xNLS in pCR8 Gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1416-pCR8-mCBPHAT_4xNLS_PUFa_2xNLS
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tccgaattca ccggtgccgc caccatgatc
      121 tttaaacccg aggagctacg ccaggcactt atgccaactc tagaagcact ctatcgacag
      181 gacccagagt ctttgccttt tcgtcagcct gtagatcctc agctcctagg aatcccagat
      241 tattttgata tagtgaagaa tcctatggac ctttctacca tcaaacgaaa gctggacaca
      301 gggcaatatc aagaaccctg gcagtatgtg gatgatgtct ggcttatgtt caacaatgcg
      361 tggctatata atcgtaaaac gtcccgtgta tataaatttt gcagtaaact tgcagaggtc
      421 tttgaacaag aaattgaccc tgtcatgcag tctcttggat attgctgtgg acgaaagtat
      481 gagttctccc cacagacttt gtgctgttac ggaaagcagc tgtgtacaat tcctcgtgat
      541 gcagcctact acagctatca gaataggtat catttctgtg agaagtgttt cacagagatc
      601 cagggcgaga atgtgaccct gggtgacgac ccttcccaac ctcagacgac aatttccaag
      661 gatcaatttg aaaagaagaa aaatgatacc ttagatcctg aaccttttgt tgactgcaaa
      721 gagtgtggcc ggaagatgca tcagatttgt gttctacact atgacatcat ttggccttca
      781 ggttttgtgt gtgacaactg tttgaagaaa actggcagac ctcggaaaga aaacaaattc
      841 agtgctaaga ggctgcagac cacacgattg gg
//
