LOCUS       sequence-136901-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector for tet-inducible expression of EGFP 
            tagged human RHOA-Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Kong YP, Carrion B, Singh RK, Putnam AJ
  TITLE     Matrix identity and tractional forces influence indirect cardiac 
            reprogramming.
  JOURNAL   Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474.
  PUBMED    24326998
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-12-11"; volume: "3:3474. doi"; pages: " 
            10.1038/srep03474"
COMMENT     Sequence Label: tetO-FUW-eGFP-RHOA-Q63L
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(57..59,60..62,63..773)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(57..59,60..62,63..773)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(102..123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(363..382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     710..731
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc tctagaatgg
       61 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      121 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      181 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      241 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      301 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      361 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      421 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      481 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      541 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      601 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      661 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      721 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggaattca
      781 tggctgccat ccggaagaaa ctggtgattg ttggtgatgg agcctgtgga a
//
