LOCUS       sequence-136902-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector for tet-inducible expression of EGFP 
            tagged human RHOA-Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Kong YP, Carrion B, Singh RK, Putnam AJ
  TITLE     Matrix identity and tractional forces influence indirect cardiac 
            reprogramming.
  JOURNAL   Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474.
  PUBMED    24326998
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-12-11"; volume: "3:3474. doi"; pages: " 
            10.1038/srep03474"
COMMENT     Sequence Label: tetO-FUW-eGFP-RHOA-Q63L
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(55..447,448..450,451..636))
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     primer_bind     complement(685..706)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcgg atcctcacaa
       61 gacaaggcac ccagattttt tcttcccacg tctagcttgc agagcagctc tcgtagccat
      121 ttcaaaaacc tctctcactc catctttggt ctttgctgaa cactccatgt acccaaaagc
      181 gccaatcctg tttgccatat ctctgccttc ttcaggtttc accggctcct gcttcatctt
      241 ggctagctcc cgccttgtgt gctcatcatt ccgaagatcc ttcttattcc caaccaggat
      301 gatgggcacg ttgggacaga aatgcttgac ttctggggtc cacttttctg ggatgttttc
      361 taaactatca gggctgtcga tggaaaaaca catcagtata acatcggtat ctgggtagga
      421 gaggggcctc aggcgatcat aatcttccag cccagctgtg tcccacaaag ccaactctac
      481 ctgctttcca tccacctcga tatctgccac atagttctca aacactgtgg gcacatacac
      541 ctctgggaac tggtccttgc tgaagactat gagcaagcat gtctttccac aggctccatc
      601 accaacaatc accagtttct tccggatggc agccatgaat tccttgtaca gctcgtccat
      661 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      721 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      781 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      841 ctcgatgt
//
