LOCUS       sequence-136905-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Clover_NLSPUFc in pCR8 gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1403-pCR8-Clover_NLSPUFc
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(115..117,118..120,121..831)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(160..181)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     768..789
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(847..865)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(848..865)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tccgaattca ccggtgccgc caccatggtg
      121 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      181 gtaaacggcc acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag
      241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      301 accaccttcg gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac
      361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag
      421 gacgacggta cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      541 gagtacaact tcaacagcca caacgtctat atcacggccg acaagcagaa gaacggcatc
      601 aaggctaact tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac
      661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      721 agccatcagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      781 gagttcgtga ccgccgccgg gattacacat ggcatggacg agctgtacaa gtctagaggg
      841 ccctattcta tagtgtcacc taaatgcgga ggcggcggta gcgggccggc cgggatcctc
      901 ccccccaaga aaaa
//
