LOCUS       sequence-136909-          65 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of sgRNA without PBS. It contains 
            extra sequences for BsaI digestion for insertion of PBS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 65)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 65)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1394-pX-sgRNA-0xPBS
FEATURES             Location/Qualifiers
     source          1..65
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgcccctg caggcagctg
       61 cgcgc
//
