LOCUS       sequence-136935-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFb_VP64 in expression vector pmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1356-pmax-NLSPUFb_VP64
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(141..290)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact
       61 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtcgaattc
      121 ttaattaatc aatcgatata taacatatcg agatcgaaat cgtccagagc atcggagccg
      181 agcatgtcca gatcaaagtc atccaatgcg tcgcttccca acatatccag gtcaaagtca
      241 tcgagggcat cagaacccag catgtcgaga tcgaaatcgt ccagcgcgtc ggcgcgcccg
      301 gatccggccg gcccccctaa gtcaacaccg ttcttcatgt agtacttctc cagcttggcc
      361 agaatgtgct tgccataggt gtacttacga agagttgcga tgtggggccg gatcttatgc
      421 atgacgatct tccgctggcc tggctccgcc acgtcaatca tcttctggac cacgtagttg
      481 gcatactggt ccttcatcat ggtgtataag gcactgtggg gaccgtcgtt catggtgcac
      541 acctcatcga tgagcacagc gcgctccgta cgtgaggcgt gagtaacaca cttctgcaca
      601 acattgtttg caaatttgtg ctgactcaat acaagtacat tgcctcggat ttctgctaca
      661 attttgcttt tatcctcagg acgaccgtgc tccagtacat gttcgattac ataacttcca
      721 tattgatcct gtaccagctg ctctgtgtgc tggtgaagct cctctaaaat agggagtgtc
      781 tggtcaggga gacagtgctc caggattctc tgaatcactc ggcagccata aggatgtgtg
      841 gataaggcaa atacctgtcc cttaaacgca tcgatgataa attgcaaaga ctggggctgt
      901 acacattcaa tgcatttctg aaccacgtga ttgccattct gatctttcac acacttcaag
      961 a
//
