LOCUS       sequence-136938-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFw_VP64 in expression vector pmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1357-pmax-NLSPUFw_VP64
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..42)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(147..296)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaacc gcgggccctc
       61 tagactcgag cggccgccac tgtgctggat atcaaccact ttgtacaaga aagctgggtc
      121 gaattcttaa ttaatcaatc gatatataac atatcgagat cgaaatcgtc cagagcatcg
      181 gagccgagca tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat atccaggtca
      241 aagtcatcga gggcatcaga acccagcatg tcgagatcga aatcgtccag cgcgtcggcg
      301 cgcccggatc cggccggccc ccctaagtca acaccgttct tcatgtagta cttctccagc
      361 ttggccagaa tgtgcttgcc ataggtgtac ttacgaagag ttgcgatgtg gggccggatc
      421 ttatgcatga cgatcttccg ctggcctggc tccgccacgt caatcatctt ctggaccacg
      481 tagttggcat actggtcctt catcatggtg tataaggcac tgtggggacc gtcgttcatg
      541 gtgcacacct cgtcgatgag cacagcgcgc tccgtacgtg aggcgtgagt aacacacttc
      601 tccacaacat tgcttgcaaa tttgtgctga ctcaatacaa gtacattgcc tcggatttct
      661 gctacaattt tgcttttatc ctcaggacga ccgtgctcca gtacatgttg gattacataa
      721 tttccatatt gatcctgtac cagctgctct gtgtgctggt gaagctcctc taaaataggg
      781 agtgtctggt cagggagaca gtgctccagg attctctgaa tcactcggca gccataagga
      841 tgtgtggata aggcaaatac ctgtccctta aacgcgtcga tgataaattg caaagactgg
      901 ggctgtacac attcaatgca tttctgaacc acgtgattgc cattctgatc tttcacacac
      961 tt
//
