LOCUS       sequence-136942-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  4xNLS_PUFa_2xNLS_mCBPHAT in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1417-pmax-4xNLS_PUFa_2xNLS_mCBPHAT
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagaatcgag accgaggaga gggttaggga taggcttacc ttcgaaccgc gggccctcta
       61 gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcga
      121 attcttaatt aactagatgt tgaggcagaa gggcacaggg catttatttt cttggcagtg
      181 tttggcgtgg tagcagcaaa gagcaatgag ctgcttgcac actgggcatc ctccattagt
      241 cttgcgcttg cagcccttgg tgtgctgcac gactcgcttc atcttctggc aagacggcag
      301 tgagcagttg gcattgcgac actggcaggc atgcaccagg gactggatgc agcgctggat
      361 gctgagacgc cgggattcct gggggctctt ggactgtggc tcaccctgac tgctgccctc
      421 atcatctagg cctagccccc acttcaccat cttatgggtg tggctctttg tgttgtagca
      481 attgatacaa aggtcatagt cctcacacac agtgcagtgc cagcgtgttt ccacatggtg
      541 tttgcactca ttgcaggtat aaacaaagcg gtcctggccc tgtgtgtgca gctccaccag
      601 catgcacaga gtggaccatt tggagcggcg taaggaagag aattcccagt gcttgtctct
      661 ggccagggtg aggaaggcat ctcgcccatc catgaggtca cagctaagca gaggatcagg
      721 gtccacgatg gggggctgag tgctgataac aggcccagca tgcagatgaa tcacaaagaa
      781 tacctccttg tgcttctcca tggtggcata cagcttctgc gacaggtcgt tggaaacatt
      841 gggcatgctg ggcttcttct tgttggcgcg gctaatgctg cttttg
//
