LOCUS       sequence-136943-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFa_VP64 in transient expression vector pmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1355-pmax-NLSPUFa_VP64
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(145..294)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tagaatcgag accgaggaga gggttaggga taggcttacc ttcgaaccgc gggccctcta
       61 gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcga
      121 attcttaatt aatcaatcga tatataacat atcgagatcg aaatcgtcca gagcatcgga
      181 gccgagcatg tccagatcaa agtcatccaa tgcgtcgctt cccaacatat ccaggtcaaa
      241 gtcatcgagg gcatcagaac ccagcatgtc gagatcgaaa tcgtccagcg cgtcggcgcg
      301 cccggatccg gccggccccc ctaagtcaac accgttcttc atgtagtact tctccagctt
      361 ggccagaatg tgcttgccat aggtgtactt acgaagagtt gcgatgtggg gccggatctt
      421 atgcatgacg atcttccgct ggcctggctc cgccacgtca atcatcttct ggaccacgta
      481 gttggcatac tggtccttca tcatggtgta taaggcactg tggggaccgt cgttcatggt
      541 gcacacctca tcgatgagca cagcgcgctc cgtacgtgag gcgtgagtaa cacacttctc
      601 cacaacattg cttgcaaatt tgtgctgact caatacaagt acattgcctc ggatttctgc
      661 tacaattttg cttttatcct caggacgacc gtgctccagt acatgttgga ttacataatt
      721 tccatattga tcctgtacca gctgctctgt gtgctggtga agctcctcta aaatagggag
      781 tgtctggtca gggagacagt gctccaggat tctctgaatc actcggcagc cataaggatg
      841 tgtggataag gcaaatacct gtcccttaaa cgcatcgatg ataaattgca aagactgggg
      901 ctgtacacat tcaatgcatt tctgaaccac gtgattgcca ttctgatctt tcacacactt
      961 c
//
