LOCUS       sequence-136944-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  4xNLS_PUFa_2xNLS_mCBPHAT in pCR8 Gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1415-pCR8-4xNLS_PUFa_2xNLS_mCBPHAT
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      790..799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat tattttttat aatgccaact ttgtacaaga aagctgggtc gaattcttaa
      121 ttaactagat gttgaggcag aagggcacag ggcatttatt ttcttggcag tgtttggcgt
      181 ggtagcagca aagagcaatg agctgcttgc acactgggca tcctccatta gtcttgcgct
      241 tgcagccctt ggtgtgctgc acgactcgct tcatcttctg gcaagacggc agtgagcagt
      301 tggcattgcg acactggcag gcatgcacca gggactggat gcagcgctgg atgctgagac
      361 gccgggattc ctgggggctc ttggactgtg gctcaccctg actgctgccc tcatcatcta
      421 ggcctagccc ccacttcacc atcttatggg tgtggctctt tgtgttgtag caattgatac
      481 aaaggtcata gtcctcacac acagtgcagt gccagcgtgt ttccacatgg tgtttgcact
      541 cattgcaggt ataaacaaag cggtcctggc cctgtgtgtg cagctccacc agcatgcaca
      601 gagtggacca tttggagcgg cgtaaggaag agaattccca gtgcttgtct ctggccaggg
      661 tgaggaaggc atctcgccca tccatgaggt cacagctaag cagaggatca gggtccacga
      721 tggggggctg agtgctgata acaggcccag catgcagatg aatcacaaag aatacctcct
      781 tgtgcttctc catggtggca tacagcttct gcgacaggtc gttggaaaca ttgggcatgc
      841 tgggcttctt cttgttggcg cggctaatgc tgcttttgtt tttgttggtc ttcttgttgt
      901 tctttttctt cgcatttttg ctgtcaccct gactgccctc aggagtctca ctcgctgca
//
