LOCUS       sequence-136945-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCBPHAT_4xNLS_PUFa_2xNLS in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1418-pmax-mCBPHAT_4xNLS_PUFa_2xNLS
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(195..215)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(219..239)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact
       61 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtcgaattc
      121 ttaattaatc aggccggccc tgatcctcca cctccgcttc cgccgccacc ggaaccgcct
      181 cccccgcggc cgcctacctt tcttttcttt ttaggatcca ctttgcgctt cttctttggg
      241 tcccctaagt caacaccgtt cttcatgtag tacttctcca gcttggccag aatgtgcttg
      301 ccataggtgt acttacgaag agttgcgatg tggggccgga tcttatgcat gacgatcttc
      361 cgctggcctg gctccgccac gtcaatcatc ttctggacca cgtagttggc atactggtcc
      421 ttcatcatgg tgtataaggc actgtgggga ccgtcgttca tggtgcacac ctcgtcgatg
      481 agcacagcgc gctccgtacg tgaggcgtga gtaacacact tctccacaac attgcttgca
      541 aatttgtgct gactcaatac aagtacattg cctcggattt ctgctacaat tttgctttta
      601 tcctcaggac gaccgtgctc cagtacatgt tggattacat aatttccata ttgatcctgt
      661 accagctgct ctgtgtgctg gtgaagctcc tctaaaatag ggagtgtctg gtcagggaga
      721 cagtgctcca ggattctctg aatcactcgg cagccataag gatgtgtgga taaggcaaat
      781 acctgtccct taaacgcgtc gatgataaat tgcaaagact ggggctgtac acattcaatg
      841 catttctgaa ccacgtgatt gccattctga tctttcacac acttcaagac atggccatct
      901 agttcccgaa ccatctcatt ctgctggtct gaaggaataa actcaaga
//
