LOCUS       sequence-136951-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9-3xGGGGS-VP64 in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC164-pmax-dCas9Master-VP64
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..42)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(147..296)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(375..395)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaacc gcgggccctc
       61 tagactcgag cggccgccac tgtgctggat atcaaccact ttgtacaaga aagctgggtc
      121 gaattcttaa ttaatcaatc gatatataac atatcgagat cgaaatcgtc cagagcatcg
      181 gagccgagca tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat atccaggtca
      241 aagtcatcga gggcatcaga acccagcatg tcgagatcga aatcgtccag cgcgtcggcg
      301 cgcccggatc cggccggccc actaccgccg ccgccggacc cgcctcctcc gctaccgccg
      361 cctccgctgg cctccacctt tctcttcttc ttggggctgt cgcctcccag ctgagacagg
      421 tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc gtccagcacc
      481 tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt gaaggcggca
      541 ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg ctctctgatg
      601 ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc gtcggccagg
      661 atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta gtgcttgtgc
      721 tgttccacaa acagctgttt ctgctcatta tcctcggggg agcccttcag cttctcatag
      781 tggctggcca ggtacaggaa gttcacatat ttggagggca gggccagttc gtttcccttc
      841 tgcagttcgc cggcagag
//
