LOCUS       sequence-136954-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFa_p65HSF1 in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1393-pmax-NLSPUFa_p65HSF1
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(138..509)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact
       61 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtcgaattc
      121 ttaattaatc aatcgatgga gacagtgggg tccttggctt tgggaggctc cgagcctgtc
      181 agcagggaga tggtggggtc ctcggcgaag ccgtcccctt cggagaagta ggagccctct
      241 cccagctcaa acagcaccgg caggtcgttg ctcccggtgt ccacggagcc ggggtccagc
      301 aggaacagcg gctgcgctgt gtagtgcacc agctgcttcc ctgaatccgg gctgctgttc
      361 tctgcctcgg gaggcctggg gggctcctgg ggagacagga gctcttggat actggccagg
      421 ctgctgtcaa ggtcaggcag gctcatgtcg ggcacggtca ccgaggggct gaacaggtcc
      481 agcagggcac tggtgtccac gctgaagccg cttccacctc ctccctgccc actagaggaa
      541 atctgtgaca gcagggcact aaagtccata tcagcgatgc ttgagaagtc ttcatctccg
      601 gacagcccat taggcaggcc gctggttccc aggggagttg gagcggggtc ggggggccgc
      661 tggctgccgg tcaccagccg ggtaatggct tcggggtact ccatcagcat tggttcggct
      721 gtactatgag acatggacac gccctgattc agcagctgct gaaactcaga gttgtccacg
      781 gaggccagat ctgtgaacac tccgggatcg gtgctgttcc ccagcagagc tcccaggtcc
      841 tcatcagcgt cgaactgcag gtgcagcaga gc
//
