LOCUS       sequence-136955-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCBPHAT_4xNLS_PUFa_2xNLS in pCR8 Gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1416-pCR8-mCBPHAT_4xNLS_PUFa_2xNLS
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(194..214)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(218..238)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgatgagc aattattttt tataatgcca actttgtaca agaaagctgg gtcgaattct
      121 taattaatca ggccggccct gatcctccac ctccgcttcc gccgccaccg gaaccgcctc
      181 ccccgcggcc gcctaccttt cttttctttt taggatccac tttgcgcttc ttctttgggt
      241 cccctaagtc aacaccgttc ttcatgtagt acttctccag cttggccaga atgtgcttgc
      301 cataggtgta cttacgaaga gttgcgatgt ggggccggat cttatgcatg acgatcttcc
      361 gctggcctgg ctccgccacg tcaatcatct tctggaccac gtagttggca tactggtcct
      421 tcatcatggt gtataaggca ctgtggggac cgtcgttcat ggtgcacacc tcgtcgatga
      481 gcacagcgcg ctccgtacgt gaggcgtgag taacacactt ctccacaaca ttgcttgcaa
      541 atttgtgctg actcaataca agtacattgc ctcggatttc tgctacaatt ttgcttttat
      601 cctcaggacg accgtgctcc agtacatgtt ggattacata atttccatat tgatcctgta
      661 ccagctgctc tgtgtgctgg tgaagctcct ctaaaatagg gagtgtctgg tcagggagac
      721 agtgctccag gattctctga atcactcggc agccataagg atgtgtggat aaggcaaata
      781 cctgtccctt aaacgcgtcg atgataaatt gcaaagactg gggctgtaca cattcaatgc
      841 atttctgaac cacgtgattg ccattctgat ctttcacaca cttcaagaca tggccatcta
      901 gttcccgaac catctcattc tgctggtctg aaggaataaa ctcaagagct ttctcgataa
      961 cacggctgcc atac
//
