LOCUS       sequence-136956-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRuby2_NLSPUFa in pCR8 gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1404-pCR8-mRuby2_NLSPUFa
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatta
       61 ttttttataa tgccaacttt gtacaagaaa gctgggtcga attcttaatt aatcacccta
      121 agtcaacacc gttcttcatg tagtacttct ccagcttggc cagaatgtgc ttgccatagg
      181 tgtacttacg aagagttgcg atgtggggcc ggatcttatg catgacgatc ttccgctggc
      241 ctggctccgc cacgtcaatc atcttctgga ccacgtagtt ggcatactgg tccttcatca
      301 tggtgtataa ggcactgtgg ggaccgtcgt tcatggtgca cacctcatcg atgagcacag
      361 cgcgctccgt acgtgaggcg tgagtaacac acttctccac aacattgctt gcaaatttgt
      421 gctgactcaa tacaagtaca ttgcctcgga tttctgctac aattttgctt ttatcctcag
      481 gacgaccgtg ctccagtaca tgttggatta cataatttcc atattgatcc tgtaccagct
      541 gctctgtgtg ctggtgaagc tcctctaaaa tagggagtgt ctggtcaggg agacagtgct
      601 ccaggattct ctgaatcact cggcagccat aaggatgtgt ggataaggca aatacctgtc
      661 ccttaaacgc atcgatgata aattgcaaag actggggctg tacacattca atgcatttct
      721 gaaccacgtg attgccattc tgatctttca cacacttcaa gacatggcca tctagttccc
      781 gaaccatctc attctgctgg tctgaaggaa taaactcaag agctttctcg ataacacggc
      841 tgccatacat ctgtagtgcc aatgacagga cgtggcctcg aatccgttct gccaaagcca
      901 gcttctgttc aagactgcca aattcaaaga acttctgaat gacgtaa
//
