LOCUS       sequence-136957-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFb pCR8 gateway donor vector for insertion of N-terminal domain
            (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in
            dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1406-pCR8-4xNLS_PUFb_2xNLS
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(192..212)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(216..236)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgatgagcaa ttatttttta taatgccaac tttgtacaag aaagctgggt cgaattctta
      121 attaatcagg ccggccctga tcctccacct ccgcttccgc cgccaccgga accgcctccc
      181 ccgcggccgc ctacctttct tttcttttta ggatccactt tgcgcttctt ctttgggtcc
      241 cctaagtcaa caccgttctt catgtagtac ttctccagct tggccagaat gtgcttgcca
      301 taggtgtact tacgaagagt tgcgatgtgg ggccggatct tatgcatgac gatcttccgc
      361 tggcctggct ccgccacgtc aatcatcttc tggaccacgt agttggcata ctggtccttc
      421 atcatggtgt ataaggcact gtggggaccg tcgttcatgg tgcacacctc gtcgatgagc
      481 acagcgcgct ccgtacgtga ggcgtgagta acacacttct gcacaacatt gtttgcaaat
      541 ttgtgctgac tcaatacaag tacattgcct cggatttctg ctacaatttt gcttttatcc
      601 tcaggacgac cgtgctccag tacatgttcg attacataac ttccatattg atcctgtacc
      661 agctgctctg tgtgctggtg aagctcctct aaaataggga gtgtctggtc agggagacag
      721 tgctccagga ttctctgaat cactcggcag ccataaggat gtgtggataa ggcaaatacc
      781 tgtcccttaa acgcgtcgat gataaattgc aaagactggg gctgtacaca ttcaatgcat
      841 ttctgaacca cgtgattgcc attctgatct ttcacacact tcaagacatg gccatctagt
      901 tcccgaacca tctcattctg ctggtctgaa ggaataaact caagagcttt ctggataaca
      961 cggcagccat acatctgtag tgccaatgac aggacgtggc ctcgaatccg tt
//
