LOCUS       sequence-136958-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFw pCR8 gateway donor vector for insertion of N-terminal domain
            (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in
            dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1407-pCR8-4xNLS_PUFw_2xNLS
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(193..213)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(217..237)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgatgagca attatttttt ataatgccaa ctttgtacaa gaaagctggg tcgaattctt
      121 aattaatcag gccggccctg atcctccacc tccgcttccg ccgccaccgg aaccgcctcc
      181 cccgcggccg cctacctttc ttttcttttt aggatccact ttgcgcttct tctttgggtc
      241 ccctaagtca acaccgttct tcatgtagta cttctccagc ttggccagaa tgtgcttgcc
      301 ataggtgtac ttacgaagag ttgcgatgtg gggccggatc ttatgcatga cgatcttccg
      361 ctggcctggc tccgccacgt caatcatctt ctggaccacg tagttggcat actggtcctt
      421 catcatggtg tataaggcac tgtggggacc gtcgttcatg gtgcacacct cgtcgatgag
      481 cacagcgcgc tccgtacgtg aggcgtgagt aacacacttc tccacaacat tgcttgcaaa
      541 tttgtgctga ctcaatacaa gtacattgcc tcggatttct gctacaattt tgcttttatc
      601 ctcaggacga ccgtgctcca gtacatgttg gattacataa tttccatatt gatcctgtac
      661 cagctgctct gtgtgctggt gaagctcctc taaaataggg agtgtctggt cagggagaca
      721 gtgctccagg attctctgaa tcactcggca gccataagga tgtgtggata aggcaaatac
      781 ctgtccctta aacgcgtcga tgataaattg caaagactgg ggctgtacac attcaatgca
      841 tttctgaacc acgtgattgc cattctgatc tttcacacac ttcaagacat ggccatctag
      901 ttcccgaacc atctcattct gctggtctga aggaataaac tcaagagctt tctggataac
      961 acggcagcca tacatctgta gtgccaatga cag
//
