LOCUS       sequence-136959-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BE1 with C-terminal NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pCMV-BE1
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..36)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatgatgacc ggttagactt tcctcttctt cttgggagaa ccaccagagt cacccccaag
       61 ctgtgacaaa tctatccgag tttcatataa tcccgtgatg gattggtgaa tcagtgtcgc
      121 gtctagcacc tccttggtag aagtgtatcg tttgcgatct atcgttgtgt caaaatactt
      181 gaatgcggct ggagcgccga ggttggtaag agtaaacaaa tggataatat tttccgcctg
      241 ctcacgtatg ggtttatccc tgtgcttgtt gtatgcgctt aatactttgt ccagattggc
      301 atcagctagg atgactctct tactgaattc cgaaatttgc tctatgattt cgtcgagata
      361 atgtttgtgc tgctcaacaa aaagttgctt ctgttcgtta tcttcaggtg aacctttcaa
      421 cttctcgtaa tgggacgcta aatacaggaa attcacgtat ttagacggta gtgcgagttc
      481 gttccccttt tgaagctctc cggcgctagc caacatccgt tttcggccat tttctaactc
      541 aaacagacta tactttggta gtttaattat gagatccttt tttacttcct tgtaaccttt
      601 cgcctcaagg aagtcgatgg ggttcttttc aaaagacgag cgctccataa tcgttatccc
      661 caataattct ttgactgact tcagtttctt ggattttccc ttctcaactt ttgccactac
      721 taggacagaa taggcaactg tagggctatc gaagccaccg tacttt
//
