LOCUS       sequence-136966-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pCMV-BE2
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..32)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(45..293)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atgaccggtt agactttcct cttcttcttg ggagaaccac cagagagcat cttaatcttg
       61 ttctcaccgt tgctatcctg tatgaccaga gcccaaggct tgtattcagg ggcgtcgcta
      121 gtcagaagca tgacattctc gtcggtgctc tcgtcgtagg cggtgtgcac gagtatatcg
      181 ctttccggct tgttcccaat gacttcttcc acctcctctg ggagcatgag gatggattcc
      241 tggataacca gttgcttacc ggtctccttt tcaataatat ctgacagatt agtagaacca
      301 ccagagtcac ccccaagctg tgacaaatct atccgagttt catataatcc cgtgatggat
      361 tggtgaatca gtgtcgcgtc tagcacctcc ttggtagaag tgtatcgttt gcgatctatc
      421 gttgtgtcaa aatacttgaa tgcggctgga gcgccgaggt tggtaagagt aaacaaatgg
      481 ataatatttt ccgcctgctc acgtatgggt ttatccctgt gcttgttgta tgcgcttaat
      541 actttgtcca gattggcatc agctaggatg actctcttac tgaattccga aatttgctct
      601 atgatttcgt cgagataatg tttgtgctgc tcaacaaaaa gttgcttctg ttcgttatct
      661 tcaggtgaac ctttcaactt ctcgtaatgg gacgctaaat acaggaaatt cacgtattta
      721 gacggtagtg cgagttcgtt cc
//
