LOCUS       sequence-136968-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pCMV-BE3
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..33)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(46..294)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 gatgaccggt tagactttcc tcttcttctt gggagaacca ccagagagca tcttaatctt
       61 gttctcaccg ttgctatcct gtatgaccag agcccaaggc ttgtattcag gggcgtcgct
      121 agtcagaagc atgacattct cgtcggtgct ctcgtcgtag gcggtgtgca cgagtatatc
      181 gctttccggc ttgttcccaa tgacttcttc cacctcctct gggagcatga ggatggattc
      241 ctggataacc agttgcttac cggtctcctt ttcaataata tctgacagat tagtagaacc
      301 accagagtca cccccaagct gtgacaaatc tatccgagtt tcatataatc ccgtgatgga
      361 ttggtgaatc agtgtcgcgt ctagcacctc cttggtagaa gtgtatcgtt tgcgatctat
      421 cgttgtgtca aaatacttga atgcggctgg agcgccgagg ttggtaagag taaacaaatg
      481 gataatattt tccgcctgct cacgtatggg tttatccctg tgcttgttgt atgcgcttaa
      541 tactttgtcc agattggcat cagctaggat gactctctta ctgaattccg aaatttgctc
      601 tatgatttcg tcgagataat gtttgtgctg ctcaacaaaa agttgcttct gttcgttatc
      661 ttcaggtgaa cctttcaact tctcgtaatg g
//
