LOCUS       sequence-136970-       15113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a renilla luciferase-YFP fusion protein under control of 
            the rat insulin 2 gene promoter (RIP7) suitable for transfection or 
            generation of transgenic animals.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Ghislain J, Fontes G, Tremblay C, Kebede MA, Poitout V
  TITLE     Dual reporter-beta-cell specific male transgenic rats for the 
            analysis of beta-cell functional mass and enrichment by flow 
            cytometry.
  JOURNAL   Endocrinology. 2015 Dec 15:en20151550.
  PUBMED    26671180
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 2015 Dec 15:en20151550."
COMMENT     Sequence Label: RIP7-RLuc-YFP-pBS
FEATURES             Location/Qualifiers
     source          1..15113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(86..105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     296..317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     580..602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     594..611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     595..611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..637
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        618..636
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     669..685
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(11640..11642,11643..11645,11646..12356)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11685..11706)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11946..11965)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12293..12314
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(12886..12902)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12887..12903)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(12931..12949)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12970..12986)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12970..12986)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12983..13005)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12994..13010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13018..13024,13025..13042,13043..13048))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13063..13084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13201..13218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13361..13949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13441..13460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14120..14911,14912..14980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14743..14762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14981..15085)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(15112..15113,1..454)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
       61 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
      361 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      421 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
      481 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
      541 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      601 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg
      661 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcagaccc attgaaaact
      721 tttcaaagga ctattaaaac ctgtgagatg ttggaaacat tcttggacac cagtaagact
      781 ctgggtaaca acttaggctc cagtgaaaat gtaagcaaag ccttaagatc caatgagaat
      841 ctgggtaggc cttaggtgcc aatgataacc ttcagaaagc tttaaatact ggtgagaatc
      901 ttagaaagaa ctttattgag gtttggtctt ttgctgtgta ttttaatgct aagtataggc
      961 ctccaagacc ctggctgctc ccaggttatt tttgattgct aaataaaaat actgacagcc
     1021 aatagctggg cagaagagac ataggctgag tttagttttc ctgggcttgg gtttgggaca
     1081 aaggttagaa gtcaagaaaa catggttctg agggctgccc aattggctaa gagcaacaca
     1141 gatgaaacat agtaacactt cagggttatt gataggaaat agatttgaat agcatagagg
     1201 ataggtatct gcccagctct tgtactgatc aaggcttatt ataaatataa aggtatgtgt
     1261 gtcttttatc cgggaactaa atggttaaag gtggggtaga aacccccagg ttggaattaa
     1321 attttctaca aaaaaaaaaa aaaaaaaaaa aaaaaaacct tagtcccctt gcattcctga
     1381 agaatggttt aggcctctga gagccttggc aaagctttaa gactcaataa gagcgcaggg
     1441 aaggtcttag accccaatga gagcctgtag tatgtttagg gctccagtga gaacctggag
     1501 aaggtcttaa gttgcagtca gaacctacag gaggttttat gatccaatga tgatgtatgg
     1561 caggttttag gtctcaatga tactttagag taagccttag atcaaatgag aatctagaga
     1621 aggcctcaaa ccccagtgac aatctgtaaa tggttttaga ctacagggag accatggaaa
     1681 gtttttcaga cccactaaaa acctgtcaag gccttatagg tcttttgaga agtcagaaaa
     1741 tattttggac ctattgagac cctgtgtaat gccttgggct ccaattagaa tctgagcaag
     1801 accttaggac atactgagaa aatggggaag gctttaaagc ccagtgacaa ccttggaagc
     1861 ccttaggccc agtgagaacc tgtagaagat tttaggcccc agtgagaacc tgtagaagat
     1921 tttgggcact gaaaagaaca tgaagaaggc tttattcctc aataagactt tggggaaagg
     1981 ccttaaatta cagtgagaac tttgggaagg cctaaggggg tcaatgtaac ttcagagaaa
     2041 gactcagatt ccaataagaa cccagaaaat gtcttaaccc caatgagaac ctctaaaagg
     2101 ttttagacca cagtgagaac atgaggaagc cattcagact tagtgaaaat atgctaaatt
     2161 ccttatagta cctgtaagaa cttgagaaaa tttaggcgcc agaatgatcc tgagtaatat
     2221 attagactct tttgtaaacc tggacaaggc tttacaaccc gttgagaacc tagagaaagt
     2281 ctttgacaac aggcaatctg ggaaggcctt tggccacgtg ataaccagca gaaggccctt
     2341 tacaacagtg gcaatccgag aagacctcag gccccattga taacttggat aagctttaag
     2401 atctagtgag accatgggaa gggccttagg ttccactgag actctgagca atgtcttagg
     2461 ctctaatgag aaccagggca aagacttaag agtcagtgag aacatgggaa agccctggga
     2521 tagccttatt ctttagtgag aacctgtaga aggttttaag ctccagaaaa ttgttaggga
     2581 aggccttaga cctaagggag actttgagaa tagctttaga tacctgtgag aacctagaga
     2641 aggccttaag gcccagtaag aacctataga agattttatg ccacagtgag aatatgagga
     2701 aggtcttcag acccattgtg tcttagtgtt ctgttgcttt ggaaagacat cgtgaccatg
     2761 tcaactcgta taatacaaag catgttactt tcaatttcag agatttagca cattatcctc
     2821 acagtgggga gcgtggtggc acacaagtag acatggtggt tgagagttct cttagcatct
     2881 gtgaacagaa ggaggagagg accactgagc ctagcttgga cttttgaaaa tcttcaagcc
     2941 tacccttagt aacacattcc ccaacactgc acacctccac acctattcta acaacgccgg
     3001 tacccctaat cctttcagta gtgacactcc atggtgacta agcattcaag atgtatgagc
     3061 ctctggaggc cattcttact caaacaccat aaagtgaaaa cccattgaaa gctttaaaga
     3121 ccctgtaaga actagaaaca gtaactttta ttcttactgc ctccatctgc taatgttggc
     3181 cttgtcctgg aagtttttag cctccataca atctagttta ggcatagaac atttttctcc
     3241 cctctgagac ttgctgctga gtgaatggct ggtgaagtga gctgttctga ctcaaaattc
     3301 ctctccacac taactgattc aatctagccc ctttatccca gcctctcctg agttgctctg
     3361 cttgtcctca tactaacttt agcaatatgt tctcgtcttc tggctccttc tcattctcct
     3421 gctcacctgt ctttacctgt acctagcttg ttctctctct aaaacatgtg tctgtacaac
     3481 tgtcccagtg caactgcctc cttcctttct tgttctctgt gctgttctct ctttaaatag
     3541 tgtccctttt ctctctcatg agaattgggc agatcctatt ctgtcaaatc tttcactttg
     3601 tctgccactc aactagatat cactttcttt ttttttttct tttctttttt tcagagctgg
     3661 gaaccgaacc cagggccttg cacttgctag gcaagcgctc taccactgag ctaaatcccc
     3721 aacccctaga tatcactttc aaattggatg cttccttgta caaactaact ttaccttcat
     3781 tgtctggtgt taacggtgca tactaagggg ggtggtgtat ccagccaggg gcattaaagg
     3841 tctatgctaa gggctgggcc acaggacaac tagaaacagg tttttttcag taaataacac
     3901 aaactcttgg ttcacagtgt ggtcaaatac cctgcaaaaa gagaaaaatt ttaggcaaca
     3961 agggaacaag tgagacaata ccctaggtac cagtgagaac atgaacaagg cttttaaaac
     4021 cagtgtgtag gcttagatac tttccaaacc tacctttgta agggttctta aacactttaa
     4081 tctcccttct agcccaccac ctaccagagg ttattaagac acagggaaag tggacctgtt
     4141 tagaaatagt tcttttgggc aattccaatc tttgttgtta ggatatcagc agttcagatt
     4201 actagcaaag cgccaaacac aaatcagcag cttcggtcca gtccactcag cggacaccac
     4261 acatgaatca gcaatgggag ttcaatccag aagaaattgt gagactccat caaccagaaa
     4321 aaactggaag gctttgccag ttagtctgag tctgtggaag catcctgaag ctgccagaat
     4381 atcatgagaa gttctttggc gagtttactc tctacaaagt cacaacaagc aaaaattggc
     4441 aaaacaatgc aaggtgaacc aatgcatgca cactgtcagc aaagactagc gaagcggtgc
     4501 acagcaaaca aaagcacgga gccttatccc cacatctgtg gtcatattta tatcctttct
     4561 ttttaaacgt cactcatctt ttcatgtgtc tgcctcagca aaacattctc acctgtgcct
     4621 gctttagtga aatattcttt cacctgtttt cccccctgta aaacctcatt atgagccaag
     4681 tctccaaaga aaccagaaat tttcaattta ccaatgagaa cgtagggcag atcttaggaa
     4741 ctagtgacaa cctgggaaat ccttaggccc caatgagaac ctgtttaaag ttttagaacc
     4801 cagtgagacc ctgaaaaaat ccttaggact cagtgagact ttgggaaact ccttgggtcg
     4861 cagtaggaac ctggagaagc tcttaagcat cagtgagaac ctggttacag agtgaggaca
     4921 tttgaaaagc ctagagaccc agtgaaaatg gtcaaaggcc tgggaaaatt tttagatatt
     4981 agtgagaccc tggtcacaca gtaggcctat tgagatcttg ggtaagcctt aggcttcaat
     5041 gagaatatag acaagacttt aaaacccagt gagaacatgg ggaaggcctt acaccccagt
     5101 gacaacctgg gaagatctta ggtccaagtg tgaatctgta gaaggtttta ggcctcagtg
     5161 agaacttgaa aaggacttta tgcctcattg agtacttaaa acccagtgca aacctgggaa
     5221 aggtcttaga ctctagtgat aaactggaga aagccctaga ccccagaaag taccaggaga
     5281 aggccttcag tcccagtgaa atgcagaaga ccttaggtcc agagagaagt cttaggactc
     5341 agtgaagacc cggtgaaagt gttaggcccc agtgcgaatg tagtatagcc attaggccat
     5401 agtgaaaatc tggggctatc cttagactac agaaagtacc agcagaaagt cttgggtccc
     5461 agtcagaaac tgagaagacc ttaggaccca ctgagaatat gggaatgatc ttaagtacaa
     5521 tgattcctgg cataagatct taatcttcta tgataccttg gataattcct cagtatctat
     5581 gagatcctgg agaaatttgc agcccccatt ataaaccttg aagggttgga gacactaaga
     5641 aagcctgggt cccagaaaga cactgggaag accttaagcc acagagaggc cccgaggaaa
     5701 accttgacct cagtgagact ctggtaacat acttggaccc tagagagatt ctggagatgt
     5761 cttatgaacc agacaaaccc tttaaaaggc tttctgttcc agaaagaccc tagtgaatgc
     5821 cttaggtccc cagaaagacc acagaaagat cttgagtcca gtgagaccct gagaaatcct
     5881 tattcttgac tgaaaccctg ggaagctctt aagctcaaat ctaaccacaa ataagaccat
     5941 aggtccaaat aatatcatag ggaaatcata aagcaggttt agatcctgga gacagctgag
     6001 acagctttag aacccactgt gactccaaga aagacctggg atcccagtga gaatctgagg
     6061 aagactcagg ccccagtcag tccctgaaga aaccctcaag ggtgaatgat aacctaggga
     6121 aggccttaga aaacttaatc ataaactagt gaaaacttta cgacccagtg agaacctggg
     6181 gaaagcctta ataagaccca gtgagaatct gggaaggcat taagactcag tgagggcatt
     6241 gcttactctg atagagtttc agaccagaga gaacgtacaa atgtcttagg ccctagggga
     6301 aggccttaga tccagtaagg acctggagaa gacttcaggc ctaattagaa cctggcaagc
     6361 tggagagata gctcagcggt taagagcact gactgcagca ctaactgctc ttccagaggt
     6421 cctgagttca aatcccagca accacatgga ggctcacaac cgtccgtcgt gagatctgat
     6481 gccctcttgt ggtgagtact catgtataac aaataaataa atcttaaaaa aaaaaaaaaa
     6541 gaaaagaaaa gaaaagaaaa cagaatctgg caaaagcatt aagccccagt gagaacctgg
     6601 aggaaagcct actaccccag tatgataaag ccttaggcct cagtcagaaa ctgagaacat
     6661 ctgggataac caaagagaat atggaaaagg ccttagaagg attgatacct gttgtaagac
     6721 cctagtcctc agtgatagat aaatccaagg tctcacagaa gtactcagcc tccattatga
     6781 ctcttgtagg actgtgggat cctggaagaa cttaggtaag gcctgtgccc cagtgagaca
     6841 ctgggaaggc cttttgccat agaaacctaa aggaaacctt tggacttagt gggaccctgc
     6901 taagacccca tttaaacctt ggtgataact attggccaca atgagaccct gaggaaaacc
     6961 tttgactaca gtaaggcttt aagaaaagcc atggacttca ataagtttct ggagaaatct
     7021 taggcctcat gagacctggc taaggcctgg gcccagtgag tttctgtggg aggtgttagg
     7081 ttgaaactgt gaagatgtcc attcagtcac tggaaagaac ttagggcctg gtaagtccat
     7141 ctgtaggttt taagcctatg tgagaccctt aggaaagttt aggcccttaa aaagtactta
     7201 gatctgggtg taacaagatg tttcagcttg taatggtatc gctactaagc ctaatgacct
     7261 gtgcctgcct ctaggactca tttagtggaa agagggactc atgccttgtg gattgtcatt
     7321 tgacttccac atttagaagg aaacaaacta atcaattaat taaaatacag atataaaaga
     7381 agaacagaga cttcaggcaa gtctgtgagc tttccttagg ccatgggaac cttagactat
     7441 caagtctagt aagacaatgg tgaaggcttc tagcccgtgg atactctaga gaaagccttc
     7501 tcccctgatg agacacttgt aacgtgtttg gatattgctg agatcctgga aaatccccat
     7561 gcttcattga ggcctcagaa aaggttggga agctacagaa gaccccgaga agtgtgggaa
     7621 catctctagg acagtgtaga agggcctcag acctcagaaa ctcttggcaa atccctgagc
     7681 cccaccaaga cctgaggaga gcctggaacc attttacaat gtaagcaggc tcttcaattg
     7741 catatgatcc tgaggaagac tagcctttcc tgagacccaa gggaagtcct taaccccact
     7801 gacgtgccaa ggctttaggt ccttcggggt tctggggaag tctgtgaatc acaagatgct
     7861 aatgagagtt tagagagagc catgaacctt gctgagtgcc tggagaaggc atgggcatca
     7921 ttaagaatct gggaccctgg gacagagagt aaaaccctct aaaaacctag agaacacaga
     7981 gcactgtgca gagagccctt cttgaagcag cacactacac tgagatctct gttctctatt
     8041 cctttgagac tttgggagaa actgtggacc acacaacaac cagaggggag gtcttagggt
     8101 tcactgagtc cctggtgcga tggcttgagt gtgagacttc cctcacagat ttgtgtgttt
     8161 aaacacttgg tctccagcat gtggtggtgt tctgggagat tgtgaaacct tttatacatg
     8221 gggtctagat gattgacaca caagagtgag tgttagggtt acaggccaac cagccctgct
     8281 tctggtctga cactgcttcc tggccagcac ctctgcaagc tcctgctgcc acagacagac
     8341 caactacagc caccatgatt tctgctccag gatgggccat gagccgaaat aaaacctctc
     8401 cttcccaaag ttgcttcttt ggaatttgtt cacagggata tgaaaggcac taactcaggc
     8461 agaaagagtt aattcctttt aagatcttgg gaagtcattc gggtccactg aggccctatg
     8521 gagggcccta agctcccttg aggccttggg aatgccatga ctttggatga gagccaggga
     8581 ggcagtgtga gccccgctga gttcccaagc agactgggtt tcactaacat ctggagaaaa
     8641 ccttagtccc tttagtactc tgggggaaac tgtaacaatt tgagagagtg gtgatatggc
     8701 aggaccatga tgcctactga gactctgagg aagattgtaa gctccaaaaa tgcctagaaa
     8761 aggtcttaac cccactgaga aactagagct ctctctgggt gcccttgcct ttaagggtac
     8821 tcaaaaaccg caaagacact ctggggaagg catctgttca gctgagtctc tagggagaga
     8881 cagagccaca atgaaactct catgggggag ggaaatgggg gacggttgct gctgagccat
     8941 gtgaagtctc aaggaagtcc ataagccaca aggagacctt gaaatagact gtgagccctg
     9001 ctgcaatctt ggggtggggc aagcctgttg cagcgttggt gtatccatga actcctttaa
     9061 gatacaagct atgtgtcatt cttcatgaaa actgcagagt cttgagctcc catgatgacc
     9121 ctgtagaggg cttaaagccc tgtgggaatg ctcagggttg tccctatatg tcactgagac
     9181 ctctgaaaaa atttaaatat cacagggaaa aaccataaag aagaccttaa tcctctttaa
     9241 gattccaagg agagccagac gtggtggtat atagctgaac ttgggagact gaggcaaaaa
     9301 gaccaagttt aagaccagcc tagctacact acaaggctcc tttctcaaaa gaaaaagctc
     9361 ctaggggggt tggaacccca atgagaccct cagcaaggcc ctctggcttc tctatggtcc
     9421 tgaggagacc tctccacact gccctggtct tcccaccctg gtcctacctg agctccttac
     9481 ctgctctcct cttgcattca cgtatcttct gctacagtag gtccacggtg gtgtcccaga
     9541 tacccggagt gtgagtggct gcagcacttt ctgggggaca agaagtaggg agcaaggggc
     9601 tcacagttca agtctggtgg ctataaagcc ctgcataggg tagagttctc gctcatgcaa
     9661 cgacaccaag ggtttttgct gtctgctcgg ggaacagggc agtaccaaat caggaacaga
     9721 aagagtcaag gatcccccaa ccactccaag tggaggctga gaaaggtttt gtagctgggt
     9781 agagtatgta ctaagagatg gagacagctg gctctgagct ctgaagcaag cacctcttat
     9841 ggagagttgc tgaccttcag gtgcaaatct aagatactac aggagaatac accatggggc
     9901 ttcagcccag ttgactcccg agtgggctat gggtttgtgg aaggagagat agaagagaag
     9961 ggacctttct tcttgaattc tgctttcctt ctacctctga gggtgagctg gggtctcagc
    10021 tgaggtgagg acacagctat cagtgggaac tgtgaaacaa cagttcaagg gacaaagtta
    10081 ctaggtcccc caacaactgc agcctcctgg ggaatgatgt ggaaaaatgc tcagccaagg
    10141 acaaagaagg cctcaccctc tctgagacaa tgtcccctgc tgtgaactgg ttcatcaggc
    10201 cacccaggag cccctattaa gactctaatt accctaaggc taagtagagg tgttgttgtc
    10261 caatgagcac tttctgcaga cctagcacca ggcaagtgtt tggaaactgc agcttcagcc
    10321 cctctggcca tctgctgatc cacccttaat gggacaaaca gcaaagtcca ggggtcaggg
    10381 ggggggtgct ttggactata aagctagtgg ggattcagta acccccagcc ctaagtgacc
    10441 agctacagtc ggaaaccatc agcaagcagg tatgtactct ccagggtggg cctggcttcc
    10501 ccagtcaaga ctccagggat ttgagggacg ctgtgggctc ttctcttaca tgtacctttt
    10561 gctagcctca accctgacta tcttccaggt caactcgaga tccatcgatt cgcgactggc
    10621 gcgccaccat gaccagcaag gtgtacgacc ccgagcagag gaagaggatg atcaccggcc
    10681 cccagtggtg ggccaggtgc aagcagatga acgtgctgga cagcttcatc aactactacg
    10741 acagcgagaa gcacgccgag aacgccgtga tcttcctgca cggcaacgcc gctagcagct
    10801 acctgtggag gcacgtggtg ccccacatcg agcccgtggc caggtgcatc atccccgatc
    10861 tgatcggcat gggcaagagc ggcaagagcg gcaacggcag ctacaggctg ctggaccact
    10921 acaagtacct gaccgcctgg ttcgagctcc tgaacctgcc caagaagatc atcttcgtgg
    10981 gccacgactg gggcgcctgc ctggccttcc actacagcta cgagcaccag gacaagatca
    11041 aggccatcgt gcacgccgag agcgtggtgg acgtgatcga gagctgggac gagtggccag
    11101 acatcgagga ggacatcgcc ctgatcaaga gcgaggaggg cgagaagatg gtgctggaga
    11161 acaacttctt cgtggagacc atgctgccca gcaagatcat gagaaagctg gagcccgagg
    11221 agttcgccgc ctacctggag cccttcaagg agaagggcga ggtgagaaga cccaccctga
    11281 gctggcccag agagatcccc ctggtgaagg gcggcaagcc cgacgtggtg cagatcgtga
    11341 gaaactacaa cgcctacctg agagccagcg acgacctgcc caagatgttc atcgagagcg
    11401 accccggctt cttcagcaac gccatcgtgg agggcgccaa gaagttcccc aacaccgagt
    11461 tcgtgaaggt gaagggcctg cacttcagcc aggaggacgc ccccgacgag atgggcaagt
    11521 acatcaagag cttcgtggag agagtgctga agaacgagca gggggatctg gcgagctctc
    11581 gagaattctc acgcgtctgc aggatatcag gtgctagatc tgttcttaag cttggcgcta
    11641 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
    11701 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
    11761 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
    11821 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
    11881 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
    11941 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
    12001 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
    12061 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
    12121 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
    12181 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
    12241 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
    12301 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggcag
    12361 ggcgatcgga actaaacgtt gaggcgtggt accgcgggcc cgggatccac cggatctaga
    12421 taaccctgca ggacaccggt atcgatggat ctggcgtctg aggctacccc tttcagttgt
    12481 tcatctcagc ggagacctca tcttcttcag tttccagcat taagcctcaa gaatggcagc
    12541 aggttctctg atctaatgtc tggctggggt tctccatctc ccacctgtat ctatattcta
    12601 tcttccacca tttataataa ttcctctgtg acaaatatca caaagttctt cctccgctgt
    12661 ggaactttct gagaatggag tcatcttctc caaactgtgt tctttttttt ttacaaataa
    12721 taaacacctt gggttgtgac gtggtttttg tgcctgagcc agttcttggt ggagaggagg
    12781 gccctgagga gtatgtgtac ttcccctatg tctgaatact gagtaccctt tacaaagtct
    12841 gggattaatc agaaatccag cctcactagg gttttaagct tatcgcgata ccgtcgacct
    12901 cgaggggggg cccggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc
    12961 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    13021 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    13081 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    13141 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    13201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    13261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    13321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    13381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    13441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    13501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    13561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    13621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    13681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    13741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    13801 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    13861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    13921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    13981 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    14041 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    14101 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    14161 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    14221 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    14281 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    14341 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    14401 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    14461 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    14521 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    14581 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    14641 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    14701 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    14761 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    14821 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    14881 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    14941 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    15001 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    15061 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
