LOCUS       sequence-136980-        7301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EXPRESs plasmid for human erythrocyte surface proteins encoding 
            ICAM4 with rat CD4d3+4-bio.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-9- 9"; volume: "480"; issue: "7378"; pages:
            "534-7"
COMMENT     Sequence Label: ICAM4-CD4d3+4-bio
FEATURES             Location/Qualifiers
     source          1..7301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    659..714
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(713..732)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2950..2968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3036..3140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3141..3209,3210..4001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3359..3378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4172..4760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4661..4680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4914..4931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5048..5069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5084..5089,5090..5107,5108..5114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5122..5138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5127..5149
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5146..5162
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5146..5162
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5433..5812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5813..6016
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5967..5987
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6013..6037
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6518..6537
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6571..6580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggat cactgcatca tattctggat gcacagaaaa tggtgtggaa
      601 tcatcgttaa gtctagacac gtgattaatt aaggatcccc cgacctcgac ctctggctaa
      661 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
      721 aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga
      781 tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg
      841 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca
      901 tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata
      961 aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc
     1021 tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa
     1081 tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc
     1141 aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt
     1201 gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat
     1261 atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta
     1321 tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg
     1381 tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca
     1441 agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc
     1501 ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg
     1561 tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt
     1621 tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa
     1681 tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg
     1741 acaagccgta aagactggat gtccatctca cacgaattta tggctatggg caacacataa
     1801 tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg
     1861 tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag
     1921 cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg
     1981 gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg
     2041 tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc
     2101 tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca
     2161 ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga
     2221 tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc
     2281 atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac
     2341 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata ggctatccta
     2401 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     2461 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     2521 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta
     2581 atagagatta gggtagtata tgctatccta atttatatct gggtagcata tactacccaa
     2641 atatctggat agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct
     2701 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     2761 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     2821 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     2881 gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa ttttcttgaa
     2941 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3001 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3361 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     3421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3661 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     3721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3841 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3961 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4861 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4921 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4981 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5041 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5101 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5161 accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg acagcttatc
     5221 atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa
     5281 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     5341 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
     5401 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
     5461 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     5521 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     5581 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     5641 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
     5701 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
     5761 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     5821 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     5881 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     5941 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
     6001 ctatataagc agagctcgtt tagtgaaccg tcagatcctc actctcttcc gcatcgctgt
     6061 ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac tcttcgcggt ctttccagta
     6121 ctcttggatc ggaaacccgt cggcctccga acggtactcc gccaccgagg gacctgagcg
     6181 agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc
     6241 aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg gtcggggttg tttctggcgg
     6301 aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgaggtga
     6361 ggtgtggcag gcttgagatc cagctgttgg ggtgagtact ccctctcaaa agcgggcatt
     6421 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc
     6481 gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc cacaggtgtc
     6541 cactcccagg tccaagttta aactgcggcc gccaccatgg gcagcctgtt ccccctgagc
     6601 ctgctgttct tcctggccgc tgcctaccct ggtgtcggca gcgccctggg cagaaggacc
     6661 aagagagccc agagccccaa gggaagccct ctggccccta gcggcaccag cgtgcctttc
     6721 tgggtccgga tgagccccga gtttgtggcc gtgcagcccg gcaagagcgt gcagctgaac
     6781 tgcagcaaca gctgccccca gccccagaac agcagcctgc ggacccctct gagacagggc
     6841 aagacactga gaggccctgg ctgggtgtcc taccagctgc tggatgtgag agcctggtct
     6901 agtctggctc actgcctggt cacctgtgcc ggcaagacca gatgggccac ctcccggatc
     6961 accgcctaca agccccccca cagcgtgatc ctggaacccc ccgtgctgaa gggccggaag
     7021 tacaccctgc ggtgccacgt gacccaggtg ttccccgtgg gctacctggt ggtcaccctg
     7081 agacacggca gcagagtgat ctacagcgag agcctggaac ggttcaccgg cctggacctg
     7141 gccaacgtga ccctgaccta cgagttcgcc gctggcccca gagatttctg gcagcccgtg
     7201 atctgccacg ccagactgaa cctggacggc ctggtcgtga gaaacagcag cgcccccatc
     7261 acactgatgc tggcctggtc cccagcccct accggcgcgc c
//
