LOCUS       sequence-137029-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Sema3E protein (truncated at cleavage site P1; ie, 
            short), C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3e(S)-AP-His
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agttctgctt taataagatc tctagattaa tggtgatggt gatgatgacc cgggtgcgcg
       61 gcgtcggtgg tgccggcggg gggcgccagg tcgcaggcgg tgtagggctc caggcaggcg
      121 gcgaaggcca tgacgtgcgc tatgaaggtc tgctcctgca cgccgtgaac caggtgcgcc
      181 tgcgggccgc gcgcgaacac cgccacgtcc tcgcctgcgt gggtctcttc gtccaggggc
      241 actgctgact gctgccgata ctcggggctc ccgctctcgc tctcggtaac atccggccgg
      301 gcgccgtcct tgagcacata gcctggaccg tttccgtata ggaggaccgt gtaggccttc
      361 ctgtcccggg ccttgccagg ggccagcccg aagatggagc tccctcgcag ggggtagcct
      421 ccgaaggaga agacgtggga gtggtcggca gtgacgaggc tcagcgtgtc ctcctcgctg
      481 gtgagctggc ccgccctctc aatggcgtcg tcgaacatga tcgtctcagt cagtgcccgg
      541 taagccctgc tttcatgatg accatggtcg atgcgaccac cctccacgaa gaggaagaag
      601 ccgcgggggt tcctgctcag caggcgcagg gcagcctctg tcatctccat cagggagggg
      661 tccagtgtgg agtctcggtg gatctcgtat ttcatgtctc caggctcaaa gagacccatg
      721 agatgggtca cagacgggtc cagggaagcc tgcatgagct cagtgcggtt
//
