LOCUS       sequence-137033-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Sema3F protein (truncated at cleavage site P3; ie, 
            long and contains a 93 nt deletion in exon 3), C-terminally fused to
            alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3f(L,-)-AP-His
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctgctttaa taagatctct agattaatgg tgatggtgat gatgacccgg gtgcgcggcg
       61 tcggtggtgc cggcgggggg cgccaggtcg caggcggtgt agggctccag gcaggcggcg
      121 aaggccatga cgtgcgctat gaaggtctgc tcctgcacgc cgtgaaccag gtgcgcctgc
      181 gggccgcgcg cgaacaccgc cacgtcctcg cctgcgtggg tctcttcgtc caggggcact
      241 gctgactgct gccgatactc ggggctcccg ctctcgctct cggtaacatc cggccgggcg
      301 ccgtccttga gcacatagcc tggaccgttt ccgtatagga ggaccgtgta ggccttcctg
      361 tcccgggcct tgccaggggc cagcccgaag atggagctcc ctcgcagggg gtagcctccg
      421 aaggagaaga cgtgggagtg gtcggcagtg acgaggctca gcgtgtcctc ctcgctggtg
      481 agctggcccg ccctctcaat ggcgtcgtcg aacatgatcg tctcagtcag tgcccggtaa
      541 gccctgcttt catgatgacc atggtcgatg cgaccaccct ccacgaagag gaagaagccg
      601 cgggggttcc tgctcagcag gcgcagggca gcctctgtca tctccatcag ggaggggtcc
      661 agtgtggagt ctcggtggat ctcgtatttc atgtctccag gctcaaagag acccatgaga
      721 tgggtcacag acgggtccag ggaagcctgc atgagctcag tgcggttcca cacataccgg
      781 gcaccctggc gcttcgccag ccattcctgc accagattct tccc
//
