LOCUS       sequence-137036-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Sema3F protein (truncated at cleavage site P1; ie, 
            short and contains no deletion in exon 3), C-terminally fused to 
            alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3f(S, +)-AP-His
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttctgcttta ataagatctc tagattaatg gtgatggtga tgatgacccg ggtgcgcggc
       61 gtcggtggtg ccggcggggg gcgccaggtc gcaggcggtg tagggctcca ggcaggcggc
      121 gaaggccatg acgtgcgcta tgaaggtctg ctcctgcacg ccgtgaacca ggtgcgcctg
      181 cgggccgcgc gcgaacaccg ccacgtcctc gcctgcgtgg gtctcttcgt ccaggggcac
      241 tgctgactgc tgccgatact cggggctccc gctctcgctc tcggtaacat ccggccgggc
      301 gccgtccttg agcacatagc ctggaccgtt tccgtatagg aggaccgtgt aggccttcct
      361 gtcccgggcc ttgccagggg ccagcccgaa gatggagctc cctcgcaggg ggtagcctcc
      421 gaaggagaag acgtgggagt ggtcggcagt gacgaggctc agcgtgtcct cctcgctggt
      481 gagctggccc gccctctcaa tggcgtcgtc gaacatgatc gtctcagtca gtgcccggta
      541 agccctgctt tcatgatgac catggtcgat gcgaccaccc tccacgaaga ggaagaagcc
      601 gcgggggttc ctgctcagca ggcgcagggc agcctctgtc atctccatca gggaggggtc
      661 cagtgtggag tctcggtgga tctcgtattt catgtctcca ggctcaaaga gacccatgag
      721 atgggtcaca gacgggtcca gggaagcctg catgagctca gtgcggttcc a
//
