LOCUS       sequence-137056-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAJ31 riboregulator encased in a permuted intron exon 
            ribozyme, which circularises the riboregulator. This mutant is 
            deficient in circularisation. Under control of a T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: pT7_circRAJ31_C873U
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(43..90)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(474..493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(475..493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      502..545
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(549..567)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcagggtgg tgacaccttg ccgttttttt gccggactgc agcaaaaaac ccctcaagac
       61 ccgtttagag gccccaaggg gttatgctag caattgagtc ctacaattta gcacgggatt
      121 gtctactaga gaggttcccc gtttagatag attacaagta taagtcacct tatactcagg
      181 cctcaattaa cccaagaaaa catgctagca gaccaataca gtgacagcgg atagggactt
      241 aattgcggat gagacgacgg taaagcttaa tattgatccc ctaggaatat taaacggtag
      301 cattatgttc agataaggtc gttaatctta ccccggaatt atatccagct gcatgtcacc
      361 atgcagagca gactatatct ccaacttgtt aaagcaagtt gtctatcgtt tcgagtcact
      421 tgaccctact ccccaaaggg ataatcgtta ggcatttatg tagaacctct agaccctata
      481 gtgagtcgta ttagaattcc agaaatcatc cttagcgaaa gctaaggatt ttttttatct
      541 gaaattctgc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      601 ttagacgtca ggtggcactc gagttgatcg ggcacgtaag aggttccaac tttcaccata
      661 atgaaataag atcactaccg ggcgtatttt
//
