LOCUS       sequence-137058-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sema4B protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema4b-AP-His
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttctgcttt aataagatct ctagattaat ggtgatggtg atgatgaccc gggtgcgcgg
       61 cgtcggtggt gccggcgggg ggcgccaggt cgcaggcggt gtagggctcc aggcaggcgg
      121 cgaaggccat gacgtgcgct atgaaggtct gctcctgcac gccgtgaacc aggtgcgcct
      181 gcgggccgcg cgcgaacacc gccacgtcct cgcctgcgtg ggtctcttcg tccaggggca
      241 ctgctgactg ctgccgatac tcggggctcc cgctctcgct ctcggtaaca tccggccggg
      301 cgccgtcctt gagcacatag cctggaccgt ttccgtatag gaggaccgtg taggccttcc
      361 tgtcccgggc cttgccaggg gccagcccga agatggagct ccctcgcagg gggtagcctc
      421 cgaaggagaa gacgtgggag tggtcggcag tgacgaggct cagcgtgtcc tcctcgctgg
      481 tgagctggcc cgccctctca atggcgtcgt cgaacatgat cgtctcagtc agtgcccggt
      541 aagccctgct ttcatgatga ccatggtcga tgcgaccacc ctccacgaag aggaagaagc
      601 cgcgggggtt cctgctcagc aggcgcaggg cagcctctgt catctccatc agggaggggt
      661 ccagtgtgga gtctcggtgg atctcgtatt tcatgtctcc aggctcaaag agacccatga
      721 gatgggtcac agacgggtcc agggaagcct gcatgagctc agtgcggttc c
//
