LOCUS       sequence-137070-        7458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Yan Q, Schwartz SL, Maji S, Huang F, Szent-Gyorgyi C, Lidke DS, 
            Lidke KA, Bruchez MP
  TITLE     Localization microscopy using noncovalent fluorogen activation by 
            genetically encoded fluorogen-activating proteins.
  JOURNAL   Chemphyschem. 2014 Mar 17;15(4):687-95. doi: 10.1002/cphc.201300757.
            Epub 2013 Nov 5.
  PUBMED    24194371
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cphc.201300757"; journalName: "Chemphyschem"; date: 
            "2014-03-17- 17"; volume: "15"; issue: "4"; pages: "687-95"
COMMENT     Sequence Label: pcDNA3.1-KozATG-dH6.2-2XG4S-actin
FEATURES             Location/Qualifiers
     source          1..7458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        619..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     773..793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     867..886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        867..885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3052..3069)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3058..3282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3328..3756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3415..3434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3625..3646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3765..3785)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3770..4099
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3950..4085
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4012..4031
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4166..4960
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4220..4239)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4830..4849
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5134..5255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5171..5190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5225..5244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5304..5320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5304..5320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5317..5339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5328..5344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5352..5358,5359..5376,5377..5382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5397..5418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5535..5552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5706..6291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5786..5805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6462..7253,7254..7322))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7085..7104
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7323..7427)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      841 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
      901 tagcgtttaa acttaagctt gccaccggat ccggcttggg gatatccacc atggaattcg
      961 gcctttctgg cctgcaagtc cagttgcaag aatctggacc tggtttggtt aaaccatctg
     1021 agacactttc cctaacctgc acagtcagtg gcgcatcaat ttcctcctcg cactattatt
     1081 ggggttggat tagacaacca cctggaaagg gcccagagtg gatagggtct atgtactact
     1141 cgggtagaac ttactataat cctgccttga agtcacgtgt taccatatcc tctgataagt
     1201 caaaaaacca attcttttta aaactcacta gcgttaccgc tgctgataca gctgtctatt
     1261 atgctgcccg tgagggccca acgcattatt atgataattc aggcccaata cctagcgatg
     1321 agtattttca acattggggc cagggcactt tagttacagt ttccggtggt ggcggctctg
     1381 gtggcggtgg cagcggcggt ggtggttccg gaggcggcgg ttctcaggtg cagctgcagg
     1441 agtcgggccc aggactggtg aagccttcgg agaccctgtc cctcacctgc actgtctctg
     1501 gtgcctccat cagcagtagt cattactact ggggctggat tcgccagccc ccagggaagg
     1561 ggcctgagtg gattgggagt atgtattata gtgggagaac gtactacaac ccggccctca
     1621 agagtcgagt caccatatca tcagacaagt cgaagaacca gttcttcttg aagttgacct
     1681 ctgtaaccgc cgcggacacg gccgtgtatt acgctgcgag ggagggaccc acacattact
     1741 atgataatag tggtccaata ccttcggatg agtatttcca gcactggggc cagggtaccc
     1801 tggtcactgt ctccgcggcc ggtctggccc atatgggagg cggtgggtct ggtggcggtg
     1861 gcagcaccgg tatggatgat gatatcgccg cgctcgtcgt cgacaacggc tccggcatgt
     1921 gcaaggccgg cttcgcgggc gacgatgccc cccgggccgt cttcccctcc atcgtggggc
     1981 gccccaggca ccagggcgtg atggtgggca tgggtcagaa ggattcctat gtgggcgacg
     2041 aggcccagag caagagaggc atcctcaccc tgaagtaccc catcgagcac ggcatcgtca
     2101 ccaactggga cgacatggag aaaatctggc accacacctt ctacaatgag ctgcgtgtgg
     2161 ctcccgagga gcaccccgtg ctgctgaccg aggcccccct gaaccccaag gccaaccgcg
     2221 agaagatgac ccagatcatg tttgagacct tcaacacccc agccatgtac gttgctatcc
     2281 aggctgtgct atccctgtac gcctctggcc gtaccactgg catcgtgatg gactccggtg
     2341 acggggtcac ccacactgtg cccatctacg aggggtatgc cctcccccat gccatcctgc
     2401 gtctggacct ggctggccgg gacctgactg actacctcat gaagatcctc accgagcgcg
     2461 gctacagctt caccaccacg gccgagcggg aaatcgtgcg tgacattaag gagaagctgt
     2521 gctacgtcgc cctggacttc gagcaagaga tggccacggc tgcttccagc tcctccctgg
     2581 agaagagcta cgagctgcct gacggccagg tcatcaccat tggcaatgag cggttccgct
     2641 gccctgaggc actcttccag ccttccttcc tgggcatgga gtcctgtggc atccacgaaa
     2701 ctaccttcaa ctccatcatg aagtgtgacg tggacatccg caaagacctg tacgccaaca
     2761 cagtgctgtc tggcggcacc accatgtacc ctggcattgc cgacaggatg cagaaggaga
     2821 tcactgccct ggcacccagc acaatgaaga tcaagatcat tgctcctcct gagcgcaagt
     2881 actccgtgtg gatcggcggc tccatcctgg cctcgctgtc caccttccag cagatgtgga
     2941 tcagcaagca ggagtatgac gagtccggcc cctccatcgt ccaccgcaaa tgcttctaat
     3001 aactcgaggc agctgtcgac tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     3061 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3121 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3181 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3241 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3301 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3361 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3421 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3481 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3541 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3601 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3661 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3721 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     3781 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3841 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3901 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3961 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4021 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4081 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4141 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4201 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4261 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4321 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4381 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4441 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4501 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4561 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4621 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4681 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4741 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4801 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4861 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4921 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4981 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5041 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5101 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5221 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5281 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6301 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6361 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6421 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6481 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6541 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6601 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6661 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6721 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6781 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6841 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6901 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6961 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7021 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7081 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7141 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7201 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7261 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7321 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7441 aaagtgccac ctgacgtc
//
