LOCUS       sequence-137077-        9863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-GATA4
FEATURES             Location/Qualifiers
     source          1..9863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2033..2052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2224..2603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2605..2803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2821..3001
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3048..3173
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3666..3899
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4084..4128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4426..4543
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4596..4614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4638..4656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4680..4698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4722..4740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4764..4782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4815..4853
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4850..4874
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4953..4972
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4953..4971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6373..6397)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6423..6464
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6474..6491
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6541..7129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6594..6614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7012..7023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7132..7148)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7133..7149)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7654..7834
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7860..7877)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7866..8090
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8136..8564
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8223..8242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8433..8454
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8573..8593)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8578..8907
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8758..8893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8820..8839
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8955..9002
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9021..9395
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9525..9646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9562..9581)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9616..9635
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9695..9711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9695..9711)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9708..9730)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9719..9735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9743..9749,9750..9767,9768..9773))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9788..9809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     2041 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     2101 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     2161 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     2221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     2821 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2881 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2941 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3001 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3061 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3121 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3181 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3241 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3301 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3361 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3421 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3481 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3541 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3601 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3661 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3721 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3781 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3841 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3901 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3961 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4021 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4081 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4141 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4201 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4261 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4321 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     4381 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     4441 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     4501 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     4561 cagcagagat ccagtttggt tatcgagttt accactccct atcagtgata gagaaaagtg
     4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4741 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     4801 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     4861 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     4921 gggaccgatc cagcctccgc ggccccgaat tgtaatacga ctcactatag ggagacccaa
     4981 gctggctagg taagcttgat caacaagttt gtacaaaaaa gcaggctcgg gagctcgcag
     5041 ggaccatgta tcagagcttg gccatggccg ccaaccacgg gccgcccccc ggtgcctacg
     5101 aggcgggcgg ccccggcgcc ttcatgcacg gcgcgggcgc cgcgtcctcg ccagtctacg
     5161 tgcccacacc gcaggtgccc tcctccgtgc tgagcctgtc ctacctccag ggcggaggcg
     5221 cgggctctgc gtccggaggc gcctcgggcg gcagctccgg tggggccgcg tctggtgcgg
     5281 ggcccgggac ccagcagggc agcccgggat ggagccaggc gggagccgac ggagccgctt
     5341 acaccccgcc gccggtgtcg ccgcgcttct ccttcccggg gaccaccggg tccctggcgg
     5401 ccgccgccgc cgctgccgcg gcccgggaag ctgcggccta cagcagtggc ggcggagcgg
     5461 cgggtgcggg cctggcgggc cgcgagcagt acgggcgcgc cggcttcgcg ggctcctact
     5521 ccagccccta cccggcttac atggccgacg tgggcgcgtc ctgggccgca gccgccgccg
     5581 cctccgccgg ccccttcgac agcccggtcc tgcacagcct gcccggccgg gccagcccgg
     5641 ccgcccgaca ccccaatctc gatatgtttg acgacttctc agaaggcaga gagtgtgtca
     5701 actgtggggc tatgtccacc ccgctctgga ggcgagatgg gacgggtcac tatctgtgca
     5761 acgcctgcgg cctctaccac aagatgaacg gcatcaaccg gccgctcatc aagcctcagc
     5821 gccggctgtc cgcctcccgc cgagtgggcc tctcctgtgc caactgccag accaccacca
     5881 ccacgctgtg gcgccgcaat gcggagggcg agcctgtgtg caatgcctgc ggcctctaca
     5941 tgaagctcca cggggtcccc aggcctcttg caatgcggaa agaggggatc caaaccagaa
     6001 aacggaagcc caagaacctg aataaatcta agacaccagc agctccttca ggcagtgaga
     6061 gccttcctcc cgccagcggt gcttccagca actccagcaa cgccaccacc agcagcagcg
     6121 aggagatgcg tcccatcaag acggagcctg gcctgtcatc tcactacggg cacagcagct
     6181 ccgtgtccca gacgttctca gtcagtgcga tgtctggcca tgggccctcc atccaccctg
     6241 tcctctcggc cctgaagctc tccccacaag gctatgcgtc tcccgtcagc cagtctccac
     6301 agaccagctc caagcaggac tcctggaaca gcctggtctt ggccgacagt cacggggaca
     6361 taatcactgc gaacccagct ttcttgtaca aagtggttga tctagagggc ccgcggttcg
     6421 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
     6481 accatcacca ttgagtttaa acccgctgat cagcctcaat tcgatatcaa gcttatcgat
     6541 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6601 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6661 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6721 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6781 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6841 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6901 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6961 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7021 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7081 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     7141 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7201 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7261 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7321 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7381 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     7441 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     7501 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     7561 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     7621 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     7681 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7741 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7801 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     7861 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     7921 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     7981 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     8041 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     8101 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     8161 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8221 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8281 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8341 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8401 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     8461 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     8521 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     8581 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8641 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8701 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8761 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8821 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     8881 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     8941 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     9001 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     9061 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     9121 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     9181 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     9241 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     9301 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     9361 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     9421 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     9481 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     9541 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9601 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     9661 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9721 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9781 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9841 cgggaaacct gtcgtgccag ctg
//
