LOCUS       sequence-137129-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by 
            RAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: cisRAJ31-GFP-LAA
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             689..700
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cggttacgaa ctccagcaga accatatgat cgcgtttctc gttcggatct ttagacagaa
       61 cgctttgcgt gctcagatag tgattgtctg gcagcagaac aggaccatca ccgattggag
      121 tgttttgctg gtagtgatca gccagctgca cgctgccatc ctccacgttg tggcgaattt
      181 taaaattcgc tttaatgcca tttttttgtt tatcggcggt gatgtaaaca ttgtggctgt
      241 taaaattgta ttccagctta tggcccagga tattgccgtc ttctttaaag tcaatgcctt
      301 tcagctcaat gcggtttacc agggtatcgc cttcaaattt cacttccgca cgcgttttgt
      361 acgtgccgtc atccttaaag gaaatcgtgc gttcctgcac atagccttcc ggcatggcgg
      421 acttgaagaa gtcatgctgc ttcatatggt ccggataacg agcaaagcac tgaacaccat
      481 aagtcagcgt cgttaccaga gtcggccaag gtaccggcag tttaccagta gtacagatga
      541 acttcagcgt cagtttacca ttagttgcgt caccttcacc ctcgccacgc acggaaaact
      601 tatgaccgtt gacatcacca tccagttcca ccagaatagg gacgacacca gtgaacagct
      661 cttcgccttt acggctagtc atattatgtt tctcctcttt atgtcgtctc accaagaggt
      721 aaagcatatc cactgtgctc agtatcttgt tatc
//
