LOCUS       sequence-137147-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial express ecDHFR protein for washing out TMP ligand for pBMN
            DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid 
            #29326).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     Wandless lab ecDHFR plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b His6-ecDHFR (WT)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             85..564
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     primer_bind     complement(615..633)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      629..676
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgatgatc agtctgattg cggcgttagc ggtagatcgc
      121 gttatcggca tggaaaacgc catgccgtgg aacctgcctg ccgatctcgc ctggtttaaa
      181 cgcaacacct taaataaacc cgtgattatg ggccgccata cctgggaatc aatcggtcgt
      241 ccgttgccag gacgcaaaaa tattatcctc agcagtcaac cgggtacgga cgatcgcgta
      301 acgtgggtga agtcggtgga tgaagccatc gcggcgtgtg gtgacgtacc agaaatcatg
      361 gtgattggcg gcggtcgcgt ttatgaacag ttcttgccaa aagcgcaaaa actgtatctg
      421 acgcatatcg acgcagaagt ggaaggcgac acccatttcc cggattacga gccggatgac
      481 tgggaatcgg tattcagcga attccacgat gctgatgcgc agaactctca cagctattgc
      541 tttgagattc tggagcggcg gtaaggatcc ggctgctaac aaagcccgaa aggaagctga
      601 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      661 cttgaggggt tttttgctga aaggaggaac tatatccgga tatcccgcaa gaggcccggc
      721 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
      781 atgagcgcat tgttagatt
//
