LOCUS       sequence-137154-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pMB41
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..141)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    550..583
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     592..608
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     592..608
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcctctgcag tcgacgggcc cgggatccga tggaatccgg aacctcctcc tccagatcct
       61 cctcctccag atcctcctcc tttgtatagt tcatccatgc catgtgtaat cccagcagct
      121 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      181 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      241 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      301 aagttctgaa aatttaaatc agattagtta gttagtaaaa tcatgtttaa acttacaact
      361 ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt atagttgtat
      421 tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt taactcgatt
      481 ctattaacaa gggtatcacc ttcaaacttg acttcagcac ctgaaaattt aaatatgtat
      541 ggttagttag aagttcctat tctctagaaa gtataggaac ttcgcggccg ctctagaact
      601 agtggatcct cagcactgtc ctgctccttg tgatggttta caaacgtaaa aagtctcttt
      661 aatacctgtt tttgcttcat attgttcagc gacagcttgc tgtacggcag gcaccagctc
      721 ttccgggatc agcgcgacga tacagccgcc aaatccgccg ccggtcatgc gtacgccacc
      781 tttgtcgcca atcacagctt tgacgatttc taccagagtg tcaatttgcg gcacggtgat
      841 ttcgaaatca tcgcgcatag aggcatgaga ctccgccatc aactcgccca tacgtttcag
      901 gtcgccttgc tccagcgcgc tggcagcttc aacgg
//
