LOCUS       sequence-137156-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pMB72
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(76..96)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(100..120)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(176..196)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    605..638
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     647..663
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     647..663
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtcgacagat ctttattcat gccattcaat cttctgagct tcgaaaatat cattaagtcc
       61 agatcctcct ccagatcctt ggaagtaaag gttctcggat ccttggaaat aaagattttc
      121 agatcctcct ccagatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
      181 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
      241 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
      301 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
      361 ctgaaaattt aaatcagatt agttagttag taaaatcatg tttaaactta caactttgat
      421 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      481 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      541 aacaagggta tcaccttcaa acttgacttc agcacctgaa aatttaaata tgtatggtta
      601 gttagaagtt cctattctct agaaagtata ggaacttcgc ggccgctcta gaactagtgg
      661 atcctcagca ctgtcctgct ccttgtgatg gtttacaaac gtaaaaagtc tctttaatac
      721 ctgtttttgc ttcatattgt tcagcgacag cttgctgtac ggcaggcacc agctcttccg
      781 ggatcagcgc gacgatacag ccgccaaatc cgccgccggt catgcgtacg ccacctttgt
      841 cgccaatcac agctttgacg atttctacca gagtgtcaat ttgcggcacg g
//
