LOCUS       sequence-137160-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Cas1 and Cas2 for the CRISPR adaptation process in 
            Escherichia coli. Has streptomycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Yosef I, Goren MG, Qimron U
  TITLE     Proteins and DNA elements essential for the CRISPR adaptation 
            process in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2012 Jul;40(12):5569-76. doi: 10.1093/nar/gks216.
            Epub 2012 Mar 8.
  PUBMED    22402487
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks216"; journalName: "Nucleic Acids Res"; date: 
            "2012-07"; volume: "40"; issue: "12"; pages: "5569-76"
COMMENT     Sequence Label: pCas1+2
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagtg
       61 cggccgctca aacaggtaaa aaagacacca accttaaacc atccaaatct accggggtac
      121 gcctgtttaa cccaaatgtc tggaactcaa atcccgtttc cgtattcgtt gcccatgcca
      181 tcactacatt gccttcttcc gccagtccag ctatttgttc ccagatcatt tcacgaattt
      241 ttgcggatac atcacctaca tatacccctg cacgtacctc caacaaccag atggctaatc
      301 tgcctcgtaa gcgcggaggt acattttcag tgaccacgac caacatactc atttcagcta
      361 ctccgatggc ctgcatctcc cagtgaaaca ggaagcggaa tggcaacagg ctgtgcatct
      421 tcaggtgggg ccggcggttg tatttctcca gcggcaagca cgtcctctat aagcggaatc
      481 aatttggcta atgttttact actgcgaaaa atatccctgc acgccaaacg gacttcccgg
      541 tccggctcac cagggttacg acgcgctatc tcaaaagctt tcggtacaac agtgtcaaat
      601 ttaatgatgt ctgcaatatc gtaaacaaag gaaagaggct ttcctgtatg cacaaaccca
      661 atagctggtg cataaccagc tgcaagtatc gccgcttcag ttacgccgta taaacaggaa
      721 gttgcagcgc taatgcattg gttgatcgta tcgccctttt cccagtcttt cggatcgtag
      781 cgacgtccat tccatgtcac gccgtattgc ttcgccagaa gtgcgtaggt tgcccgcacg
      841 cgactgcctt ctatacctct gagttgctct acggagcgcc gggca
//
