LOCUS       sequence-137169-       10119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E
  TITLE     A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive 
            Bacterial Immunity.
  JOURNAL   Science. 2012 Aug 17;337(6096):816-21. doi: 10.1126/science.1225829.
            Epub 2012 Jun 28.
  PUBMED    22745249
REFERENCE   2  (bases 1 to 10119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1225829"; journalName: "Science"; date: 
            "2012-08-17- 17"; volume: "337"; issue: "6096"; pages: "816-21"
COMMENT     Sequence Label: pMJ824
FEATURES             Location/Qualifiers
     source          1..10119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(70..89)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     230..252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(246..437)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1246..2328)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2290..2309
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2329..2406)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2612..2631
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2715..2734
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2715..2733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2734..2758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2773..2795
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2808..2825
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2829..3482
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             3501..3521
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7698..7715
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7768..7786)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7782..7829
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7866..8321
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7953..7972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8163..8184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8413..9228)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8413..9228)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9136..9155
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9220..9239
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9350..9938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9839..9858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10092..10109
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
      121 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
      181 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      241 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
      301 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
      361 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
      421 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
      481 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      541 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      601 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
      661 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
      721 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
      781 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
      841 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
      901 cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
      961 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     1021 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     1081 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     1141 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     1201 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     1261 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1321 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     1381 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     1441 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     1501 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     1561 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     1621 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     1681 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     1741 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     1801 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     1861 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     1921 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     1981 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     2041 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     2101 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     2161 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     2221 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     2281 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     2341 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     2401 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     2461 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     2521 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     2581 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     2641 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
     2701 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
     2761 tctagaaata attttgttta actttaagaa ggagatatac catgaaacat caccatcacc
     2821 atcaccccat gagccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc
     2881 gacttctttt ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag
     2941 gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt
     3001 atattgatgg tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca
     3061 agcacaacat gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag
     3121 cggttttgga tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc
     3181 tcaaagttga ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat
     3241 gtcataaaac atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg
     3301 ctcttgatgt tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt
     3361 gttttaaaaa acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt
     3421 atatagcatg gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa
     3481 aaggatctgg cagtggttct gagaatcttt attttcaggg cgccatggct aagccatact
     3541 caattggact tgatattgga acgaatagtg ttggatgggc tgtaacaact gataattaca
     3601 aggttccgtc taaaaaaatg aaagtcttag gaaatacgag taaaaagtat atcaaaaaga
     3661 acctgttagg tgtattactc tttgactctg gaatcacagc agaaggaaga agattgaagc
     3721 gtactgcaag aagacgttat actagacgcc gtaatcgtat cctttatttg caggaaattt
     3781 ttagcacaga gatggctaca ttagatgatg ctttctttca aagacttgac gattcgtttt
     3841 tagttcctga tgataaacgt gatagtaagt atccgatatt tggaaactta gtagaagaaa
     3901 aagcctatca tgatgaattt ccaactatct atcatttaag gaaatattta gcagatagta
     3961 ctaaaaaagc agatttgcgt ctagtttatc ttgcattggc tcatatgatt aaatatagag
     4021 gtcacttctt aattgaagga gagtttaatt caaaaaataa tgatattcag aagaattttc
     4081 aagacttttt ggacacttat aatgctattt ttgaatcgga tttatcactt gagaatagta
     4141 aacaacttga ggaaattgtt aaagataaga ttagtaaatt agaaaagaaa gatcgtattt
     4201 taaaactctt ccctggggag aagaattcgg ggattttttc agagtttcta aagttgattg
     4261 taggaaatca agctgatttt aggaaatgtt ttaatttaga cgaaaaagcc tccttacatt
     4321 tttccaaaga aagctatgat gaagatttag agactttgtt aggttatatt ggagatgatt
     4381 acagtgatgt ctttctcaaa gcaaagaaac tttatgatgc tattctttta tcgggttttc
     4441 tgactgtaac tgataatgag acagaagcac ctctctcttc tgctatgata aagcgatata
     4501 atgaacacaa agaagattta gcgttactaa aggaatatat aagaaatatt tcactaaaaa
     4561 cgtataatga agtatttaaa gatgacacca aaaatggtta tgctggttat attgatggaa
     4621 aaacaaatca ggaagatttc tacgtatatc taaaaaaact attggctgaa tttgaaggtg
     4681 cggattattt tcttgaaaaa attgatcgag aagatttttt gagaaagcaa cgtacatttg
     4741 acaatggttc gataccatat cagattcatc ttcaagaaat gagagcaatt cttgataagc
     4801 aagctaaatt ttatcctttc ttggctaaaa ataaagaaag aatcgagaag attttaacct
     4861 tccgaattcc ttattatgta ggtccacttg cgagagggaa tagtgatttt gcctggtcaa
     4921 taagaaaacg aaatgaaaaa attacacctt ggaattttga ggacgttatt gacaaagaat
     4981 cttcggcaga ggccttcatt aatcgaatga ctagttttga tttgtatttg ccagaagaga
     5041 aggtacttcc aaagcatagt ctcttatacg aaacttttaa tgtatataat gaattaacaa
     5101 aagttagatt tattgccgaa agtatgagag attatcaatt tttagatagt aagcagaaga
     5161 aagatattgt tagactttat tttaaagata aaaggaaagt tactgataag gatattattg
     5221 aatatttaca tgcaatttat gggtatgatg gaattgaatt aaaaggcata gagaaacagt
     5281 ttaattctag tttatctact tatcacgatc ttttaaatat tattaatgat aaagagtttt
     5341 tggatgatag ttcaaatgaa gcgattatcg aagaaattat ccatactttg acaatttttg
     5401 aagatagaga gatgataaaa caacgtcttt caaaatttga gaatatattc gataaatccg
     5461 ttttgaaaaa gttatctcgt agacattaca ctggctgggg taagttatct gctaagctta
     5521 ttaatggtat tcgagatgaa aaatctggta atactattct tgattactta attgatgatg
     5581 gtatttctaa ccgtaatttc atgcaactta ttcacgatga tgctctttct tttaaaaaga
     5641 agatacagaa agcacaaatt attggtgacg aagataaagg taatattaaa gaggtcgtta
     5701 agtctttgcc aggtagtcct gcgattaaaa aaggtatttt acaaagcata aaaattgtag
     5761 atgaattggt caaagtaatg ggaggaagaa aacccgagtc aattgttgtt gagatggctc
     5821 gtgaaaatca atataccaat caaggtaagt ctaattccca acaacgcttg aaacgtttag
     5881 aaaaatctct caaagagtta ggtagtaaga tacttaagga aaatattcct gcaaaacttt
     5941 ctaaaataga caataacgca cttcaaaatg atcgacttta cttatactat cttcaaaatg
     6001 gaaaagatat gtataccgga gatgatttag atattgatag attaagtaat tatgatattg
     6061 atcatattat tcctcaagct tttttgaaag ataattctat tgacaataaa gtacttgttt
     6121 catctgctag taaccgtggt aaatcagatg atgttccaag tttagaggtt gtcaaaaaaa
     6181 gaaagacatt ttggtatcaa ttattgaaat caaaattaat ttctcaacga aaatttgata
     6241 atctgacaaa agctgaacgg ggaggattgt cacctgagga caaagctggt tttattcaac
     6301 gccagttggt tgaaacacgt caaataacaa aacatgtagc tcgtttactt gatgagaaat
     6361 ttaataataa aaaagatgaa aataatagag cggtacgaac agtaaaaatt attaccttga
     6421 aatctacctt agtttctcaa tttcgtaagg attttgaact ttataaagtt cgtgaaatca
     6481 atgattttca tcatgctcat gatgcttact tgaatgccgt tgtagcaagt gctttactta
     6541 agaaataccc taaactagag ccagaatttg tgtacggtga ttatccaaaa tacaatagtt
     6601 ttagagaaag aaagtccgct acagaaaagg tatatttcta ttcaaatatc atgaatatct
     6661 ttaaaaaatc tatttcttta gctgatggta gagttattga aagaccactt attgaggtaa
     6721 atgaggagac cggcgaatcc gtttggaata aagaatctga tttagcaact gtaaggagag
     6781 tactctctta tccgcaagta aatgttgtga aaaaagttga ggaacagaat cacggattgg
     6841 atagaggaaa accaaaggga ttgtttaatg caaatctttc ctcaaagcca aaaccaaata
     6901 gtaatgaaaa tttagtaggt gctaaagagt atcttgaccc caaaaagtat ggggggtatg
     6961 ctggaatttc taattctttt actgttcttg ttaaagggac aattgaaaaa ggtgctaaga
     7021 aaaaaataac aaatgtacta gaatttcaag gtatttctat tttagatagg attaattata
     7081 gaaaagataa acttaatttt ttacttgaaa aaggttataa agatattgag ttaattattg
     7141 aactacctaa atatagttta tttgaacttt cagatggttc acgtcgtatg ttggctagta
     7201 ttttgtcaac gaataataag aggggagaga ttcacaaagg aaatcagatt tttctttcac
     7261 agaagtttgt gaaattactt tatcatgcta agagaataag taacacaatt aatgagaatc
     7321 atagaaaata tgttgagaac cataaaaaag agtttgaaga attattttac tacattcttg
     7381 agtttaatga gaattatgtt ggagctaaaa agaatggtaa actcttaaac tctgcctttc
     7441 aatcttggca aaatcatagt atagatgaac tctgtagtag ttttatagga cctaccggaa
     7501 gtgaaagaaa ggggctattt gaattaacct ctcgtggaag tgctgctgat tttgaatttt
     7561 taggtgttaa aattccaagg tatagagact ataccccatc atccctatta aaagatgcca
     7621 cacttattca tcaatctgtt acaggcctct atgaaacacg aatagacctt gccaaactag
     7681 gagagggtta actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
     7741 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     7801 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
     7861 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7921 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7981 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8041 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8101 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8161 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8221 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8281 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     8341 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8401 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     8461 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     8521 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     8581 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     8641 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
     8701 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     8761 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     8821 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     8881 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     8941 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     9001 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     9061 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     9121 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     9181 atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     9241 attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt
     9301 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9361 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9421 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9481 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9541 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9601 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9661 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9721 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9781 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9841 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9901 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9961 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10021 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10081 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
