LOCUS       sequence-137173-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: 
            CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2372
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atctactgcg aaagcagcgg actttcgaca acggtagcat tccacatcaa atccacttag
       61 gcgaattgca tgctatactt agaaggcagg aggattttta tccgttcctc aaagacaatc
      121 gtgaaaagat tgagaaaatc ctaacctttc gcatacctta ctatgtggga cccctggccc
      181 gagggaactc tcggttcgca tggatgacaa gaaagtccga agaaacgatt actccatgga
      241 attttgagga agttgtcgat aaaggtgcgt cagctcaatc gttcatcgag aggatgacca
      301 actttgacaa gaatttaccg aacgaaaaag tattgcctaa gcacagttta ctttacgagt
      361 atttcacagt gtacaatgaa ctcacgaaag ttaagtatgt cactgagggc atgcgtaaac
      421 ccgcctttct aagcggagaa cagaagaaag caatagtaga tctgttattc aagaccaacc
      481 gcaaagtgac agttaagcaa ttgaaagagg actactttaa gaaaattgaa tgcttcgatt
      541 ctgtcgagat ctccggggta gaagatcgat ttaatgcgtc acttggtacg tatcatgacc
      601 tcctaaagat aattaaagat aaggacttcc tggataacga agagaatgaa gatatcttag
      661 aagatatagt gttgactctt accctctttg aagatcggga aatgattgag gaaagactaa
      721 aaacatacgc tcacctgttc gacgataagg ttatgaaaca gttaaagagg cgtcgctata
      781 cgggctgggg agccttgtcg cggaaactta tcaacgggat aagagaca
//
