LOCUS       sequence-137174-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of EGFP in human and mouse neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  TITLE     Maletic-Savatic LAB, unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      441..450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(447..449,450..452,453..965)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(492..513)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(753..772)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca
       61 aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc
      121 actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg
      181 ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc
      241 cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg
      301 cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc
      361 tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgagaaggta
      421 ccggatcctc tagagtcgac gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      481 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      541 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      601 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      661 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      721 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      781 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      841 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      901 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      961 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacacccc
//
