LOCUS       sequence-137176-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of EGFP in human and mouse neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     Maletic-Savatic LAB, unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    210..798
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             316..327
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     725..745
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(868..889)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcttcccaac ttgccccttg ctccatacca cccccctcca ccccataata ttatagaagg
       61 acacctagtc agacaaaatg atgcaactta attttattag gacaaggctg gtgggcactg
      121 gagtggcaac ttccagggcc aggagaggca ctggggaggg gtcacaggga tgccacccgt
      181 agatctctcg agcagcgctc ggtatcgatg cggggaggcg gcccaaaggg agatccgact
      241 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
      301 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
      361 ccaggtggca acataggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
      421 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
      481 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
      541 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
      601 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
      661 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
      721 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
      781 gtaatccaga ggttgattat cgataagctt gctgcagaat tcttacttgt acagctcgtc
      841 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      901 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
      961 cacggggccg tcgccgatgg gg
//
