LOCUS       sequence-137185-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BAZ2A
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(503..520)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             539..561
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    576..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(600..619)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(601..619)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(703..722)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atcctcgagt cattaaacct gctgtgccag acaaacggta caaaaccaat
       61 caccttccgg aactgcttcc attttcggac gatgacaata gatatgacaa ccacgatcac
      121 aaccatcaca cagcagcaga aattcatcat tatcgccttt gcgacaaacc agacaggtaa
      181 ctttattcac gctcatatga ccaccggttt gttcctgata aacttcaatc acatcttctt
      241 cctccattcc cagttctttt ggagtatgat tatcagcaat tctctgaccc tcaaagagaa
      301 acctgagtga attcattgga acaccctgtc tttgacagta tgattctttg agtttcttga
      361 gatgtgttgt cattttcact ttgaagtgaa tctcactgct atcctgtcca atgactttga
      421 gtttaatata ttcaccttcc ttcttatccc ccaagtcctc agttgaaggt tttgcctcct
      481 ggtcagagcc gctgccgctg ccgtgatgat gatgatgatg gctgctgcac atggtatatc
      541 tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc
      601 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca
      661 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
      721 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
      781 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
      841 ttgcgg
//
