LOCUS       sequence-137189-        9356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9356)
  TITLE     HDAC4 Plasmids
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HDAC4.3SA-FLAG
FEATURES             Location/Qualifiers
     source          1..9356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        23..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        403..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     557..577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     651..670
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        651..669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          706..1278
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             4651..4674
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4697..4715)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4698..4715)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4735..4752)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4741..4965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5011..5439
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5098..5117)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5308..5329
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5448..5468)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5453..5782
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5633..5768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5695..5714
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5849..6643
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5903..5922)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6513..6532
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6817..6938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6854..6873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6908..6927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6987..7003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6987..7003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7000..7022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7011..7027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7035..7041,7042..7059,7060..7065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7080..7101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7218..7235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7389..7977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7469..7488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8148..8939,8940..9008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8771..8790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9009..9113)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9188..9207)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      601 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
      661 cactataggg agacccaagc tagagtcgat cctgagaact tcagggtgag tttggggacc
      721 cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt
      781 ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa
      841 ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca
      901 ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact
      961 tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag
     1021 ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa
     1081 ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac
     1141 aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt
     1201 gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc
     1261 ttcttctttt tcctacagct cctgggcaac gtgctggttt gtgctgtcga ccccaagctt
     1321 ggcccgggcg gatccactag taacggccgc cagtgtgctg gaattctgca gatatccatc
     1381 acactggcgg ccgccatgag ctcccaaagc catccagatg gactttctgg ccgagaccag
     1441 ccagtggagc tgctgaatcc tgcccgcgtg aaccacatgc ccagcacggt ggatgtggcc
     1501 acggcgctgc ctctgcaagt ggccccctcg gcagtgccca tggacctgcg cctggaccac
     1561 cagttctcac tgcctgtggc agagccggcc ctgcgggagc agcagctgca gcaggagctc
     1621 ctggcgctca agcagaagca gcagatccag aggcagatcc tcatcgctga gttccagagg
     1681 cagcacgagc agctctcccg gcagcacgag gcgcagctcc acgagcacat caagcaacaa
     1741 caggagatgc tggccatgaa gcaccagcag gagctgctgg aacaccagcg gaagctggag
     1801 aggcaccgcc aggagcagga gctggagaag cagcaccggg agcagaagct gcagcagctc
     1861 aagaacaagg agaagggcaa agagagtgcc gtggccagca cagaagtgaa gatgaagtta
     1921 caagaatttg tcctcaataa aaagaaggcg ctggcccacc ggaatctgaa ccactgcatt
     1981 tccagcgacc ctcgctactg gtacgggaaa acgcagcaca gttcccttga ccagagttct
     2041 ccaccccaga gcggagtgtc gacctcctat aaccacccgg tcctgggaat gtacgacgcc
     2101 aaagatgact tccctcttag gaaaacagct gctgaaccga atctgaaatt acggtccagg
     2161 ctaaagcaga aagtggccga aagacggagc agccccctgt tacgcaggaa agacgggcca
     2221 gtggtcactg ctctaaaaaa gcgtccgttg gatgtcacag actccgcgtg cagcagcgcc
     2281 ccaggctccg gacccagctc acccaacaac agctccggga gcgtcagcgc ggagaacggt
     2341 atcgcgcccg ccgtccccag catcccggcg gagacgagtt tggcgcacag acttgtggca
     2401 cgagaaggct cggccgctcc acttcccctc tacacatcgc catccttgcc caacatcacg
     2461 ctgggcctgc ctgccaccgg cccctctgcg ggcacggcgg gccagcagga caccgagaga
     2521 ctcacccttc ccgccctcca gcagaggctc tcccttttcc ccggcaccca cctcactccc
     2581 tacctgagca cctcgccctt ggagcgggac ggaggggcag cgcacagccc tcttctgcag
     2641 cacatggtct tactggagca gccaccggca caagcacccc tcgtcacagg cctgggagca
     2701 ctgcccctcc acgcacagtc cttggttggt gcagaccggg tgtccccctc catccacaag
     2761 ctgcggcagc accgcccact ggggcggacc caggcggccc cgctgcccca gaacgcccag
     2821 gctctgcagc acctggtcat ccagcagcag catcagcagt ttctggagaa acacaagcag
     2881 cagttccagc agcagcaact gcagatgaac aagatcatcc ccaagccaag cgagccagcc
     2941 cggcagccgg agagccaccc ggaggagacg gaggaggagc tccgtgagca ccaggctctg
     3001 ctggacgagc cctacctgga ccggctgccg gggcagaagg aggcgcacgc acaggccggc
     3061 gtgcaggtga agcaggagcc cattgagagc gatgaggaag aggcagagcc cccacgggag
     3121 gtggagccgg gccagcgcca gcccagtgag caggagctgc tcttcagaca gcaagccctc
     3181 ctgctggagc agcagcggat ccaccagctg aggaactacc aggcgtccat ggaggccgcc
     3241 ggcatccccg tgtccttcgg cggccacagg cctctgtccc gggcgcaggc gtcacccgcg
     3301 tctgccacct tccccgtgtc tgtgcaggag ccccccacca agccgaggtt cacgacaggc
     3361 ctcgtgtatg acacgctgat gctgaagcac cagtgcacct gcgggagtag cagcagccac
     3421 cccgagcacg ccgggaggat ccagagcatc tggtcccgcc tgcaggagac gggcctccgg
     3481 ggcaaatgcg agtgcatccg cggacgcaag gccaccctgg aggagctaca gacggtgcac
     3541 tcggaagccc acaccctcct gtatggcacg aaccccctca accggcagaa actggacagt
     3601 aagaaacttc taggctcgct cgcctccgtg ttcgtccggc tcccttgcgg tggtgttggg
     3661 gtggacagtg acaccatatg gaacgaggtg cactcggcgg gggcagcccg cctggctgtg
     3721 ggctgcgtgg tagagctggt cttcaaggtg gccacagggg agctgaagaa tggctttgct
     3781 gtggtccgcc cccctggaca ccatgcggag gagagcacgc ccatgggctt ttgctacttc
     3841 aactccgtgg ccgtggcagc caagcttctg cagcagaggt tgagcgtgag caagatcctc
     3901 atcgtggact gggacgtgca ccatggaaac gggacccagc aggctttcta cagcgaccct
     3961 agcgtcctgt acatgtccct ccaccgctac gacgatggga acttcttccc aggcagcggg
     4021 gctcctgatg aggtgggcac agggcccggc gtgggtttca acgtcaacat ggctttcacc
     4081 ggcggcctgg acccccccat gggagacgct gagtacttgg cggccttcag aacggtggtc
     4141 atgccgatcg ccagcgagtt tgccccggat gtggtgctgg tgtcatcagg cttcgatgcc
     4201 gtggagggcc accccacccc tcttgggggc tacaacctct ccgccagatg cttcgggtac
     4261 ctgacgaagc agctgatggg cctggctggc ggccggattg tcctggccct cgagggaggc
     4321 cacgacctga ccgccatttg cgacgcctcg gaagcatgtg tttctgcctt gctgggaaac
     4381 gagcttgatc ctctcccaga aaaggtttta cagcaaagac ccaatgcaaa cgctgtccgt
     4441 tccatggaga aagtcatgga gatccacagc aagtactggc gctgcctgca gcgcacaacc
     4501 tccacagcgg ggcgttctct gatcgaggct cagacttgcg agaacgaaga agccgagacg
     4561 gtcaccgcca tggcctcgct gtccgtgggc gtgaagcccg ccgaaaagag accagatgag
     4621 gagcccatgg aagaggagcc gcccctgggg gattataaag atgatgatga taaataagaa
     4681 ttctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     4741 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4801 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4861 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4921 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4981 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5041 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5101 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5161 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5221 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5281 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5341 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5401 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5461 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5521 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5581 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5641 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5701 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5761 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5821 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5881 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5941 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6001 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6061 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6121 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6181 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6241 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6301 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6361 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6421 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6481 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6541 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6601 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6661 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6721 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6781 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6841 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6901 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6961 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7021 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7081 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7141 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7201 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7261 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7321 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7381 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7441 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7501 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7561 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7621 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7681 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7741 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7801 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7861 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7921 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7981 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8041 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8101 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8161 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8221 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8281 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8341 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8401 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8461 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8521 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8581 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8641 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8701 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8761 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8821 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8881 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8941 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9001 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9061 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9121 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     9181 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9241 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     9301 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgat
//
