LOCUS       sequence-137190-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPIN4
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(745..765)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(790..807)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcaa ggagttttca ccaaaccata
      121 gacataaatg tgaatatcat catcaaactt aatgaagtaa acagatggct tcgccaccac
      181 ttgatgtata aaaatgccag ttctcttgga cccgtcatct ttggcatgct ccacctgctt
      241 gcccacgaga ctgtcgacca cctctccagg ctcctgttct gctgtaggga aatagtagtt
      301 ggaatctgga ataatgcgta agtcaccatc tttgtagtca tcaagcagcg tgtacatata
      361 gagaacagga tctttctcgt aggtgatgta aaaccaagta tccatcacag gagctcgcgc
      421 caggaccata cccttccatt catccttggt accatgttca ccttcaaaca catgctccac
      481 tgccttgcca atcagggaat ctgccagtcg tgaatcgata cgaggagttg gcactctctc
      541 aggaaggatc tctagcgcta aaactctctt atcgcggtgc agttctagtc catacacact
      601 atctttgcca tcatatttaa tgatgtaaag agtgggcttc acggaaacct gctcgagcac
      661 agtacccttc cactgctcca ctggctcgtt gccttccttc cagccgtgtt gaatgcggca
      721 gcccacgatg ttcctacgag tcatggattg gaagtacagg ttctcggtac ccagatctac
      781 accagaagaa tgatgatgat gatggtgcat atgtatatct ccttcttaaa g
//
