LOCUS       sequence-137192-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SLX4
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(499..519)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(544..561)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             572..594
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    609..633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(633..652)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(634..652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gcttgtcgac ggagctcgaa ttcggatccg tatccacctt tactgtcact ctgagtcagt
      121 ggcaataggc acctgttcgc acaggtgaac gagctcactc acgccaaacc tgtgggccag
      181 ggagctcagc tcagagctaa ggccaggagg aaggccagtg tccgcagtgt agagatagtg
      241 caggaacgtg cgggcggcct cggtgctcac gtcacccagc aggacacgct gggtttcaac
      301 cccgtcctcg atagcggaga agccttcatt gttcacatac tggatgagga gcgggcatcg
      361 ggcataaagc acgaacttgt gggcgtaaag cacctccccg ctgtccgtct gaaactggac
      421 atcactcagg tgtgggttat tgaccatggc gccaaagtca gcaaccagca gcccgaggga
      481 gagcaaggtg cggcccatgg attggaagta caggttctcg gtacccagat ctacaccaga
      541 agaatgatga tgatgatggt gcatatgtat atctccttct taaagttaaa caaaattatt
      601 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      661 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccaca
//
