LOCUS       sequence-137198-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAK1
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..98)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtatcaatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgct gcaattgaag tctctgtggt gggaatggga
      181 tctgtcagtc tggcctttgg ctgggtcttt tttgcagctg cctcactggc tttcactggt
      241 tcaggaagct ttgcaggaat gggagagttc tgtacatttg gaattgggca ctctttcttg
      301 agtagcttaa atgagaagta ggacacctgg taaatatccg gccttttgtc agggtctggt
      361 tccaacatat acctaattag gcagtgcatg tcttgagaat atcgagaatt atcaggaatt
      421 gtgaagtttc catcacaaat tgccacctga ctttccccaa atggcaaagt gaagtagcat
      481 aatttataca acaaacatcc aagagcccaa atgtctgcct tcgtagtgat gattttgcca
      541 ctgtacaggt tgaccatttc tggtgctcga taggacagcg ttgtgtattt cttaatctca
      601 tcttctactg cattgactcc ctcagtttgt ggattctgga atttgttggt ggcgcttcca
      661 aagtcacaca ggacatagtg gcctcggtca tgcaagagga tgttttcaac cttcaggtcc
      721 cggtggataa taggagtttt gcactgatgc aggcgggcaa cagcttcaca ggtatcacaa
      781 aa
//
