LOCUS       sequence-137204-        7123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Hirai H, Tani T, Katoku-Kikyo N, Kellner S, Karian P, Firpo M, Kikyo
            N
  TITLE     Radical acceleration of nuclear reprogramming by chromatin 
            remodeling with the transactivation domain of MyoD.
  JOURNAL   Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684.
  PUBMED    21732495
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/stem"; 
            journalName: "Stem Cells"; date: "2011-09"; volume: "29"; issue: 
            "9"; pages: "1349-61"
COMMENT     Sequence Label: pMXs-hM₃O-IP
FEATURES             Location/Qualifiers
     source          1..7123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(986..1777,1778..1846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1609..1628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1847..1951)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1981..2574
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2637..2994
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3053..3469
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3399..3421
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3437..3453
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3479..3853
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     3791..3807
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    5233..5800
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5250..5802
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5394..5411)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5621..5640
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5814..6416
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MVTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6313..6333
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6587..7056
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca atctaaagta
      961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat
     1981 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     2041 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
     2101 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     2161 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     2221 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     2281 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     2341 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     2401 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
     2461 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     2521 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
     2581 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     2641 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
     2701 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     2761 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
     2821 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
     2881 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     2941 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     3001 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     3061 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     3121 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     3181 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     3241 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     3301 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     3361 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     3421 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     3481 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     3541 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     3601 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     3661 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     3721 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     3781 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     3841 atcctctaga ctgccggatc tagctagtta attaaggatc ccagtgtggt ggtacgggaa
     3901 ttcgccatgg agctactgtc gccaccgctc cgcgacgtag acctgacggc ccccgacggc
     3961 tctctctgct cctttgccac aacggacgac ttctatgacg acccgtgttt cgactccccg
     4021 gacctgcgct tcttcgaaga cctggacccg cgcctgatgc acgtgggcgc gctcctgaaa
     4081 cccgaagagc acatggcggg acacctggct tcggatttcg ccttctcgcc ccctccaggt
     4141 ggtggaggtg atgggccagg ggggccggag ccgggctggg ttgatcctcg gacctggcta
     4201 agcttccaag gccctcctgg agggccagga atcgggccgg gggttgggcc aggctctgag
     4261 gtgtggggga ttcccccatg ccccccgccg tatgagttct gtggggggat ggcgtactgt
     4321 gggccccagg ttggagtggg gctagtgccc caaggcggct tggagacctc tcagcctgag
     4381 ggtgaagcag gagtcggggt ggagagcaac tccgatgggg cctccccgga gccctgcacc
     4441 gtcacccctg gtgccgtgaa gctggagaag gagaagctgg agcaaaaccc ggaggagtcc
     4501 caggacatca aagctctgca gaaagaactc gagcaatttg ccaagctcct gaagcagaag
     4561 aggatcaccc tgggatatac acaggccgat gtggggctca ccctgggggt tctatttggg
     4621 aaggtattca gccaaacgac catctgccgc tttgaggctc tgcagcttag cttcaagaac
     4681 atgtgtaagc tgcggccctt gctgcagaag tgggtggagg aagctgacaa caatgaaaat
     4741 cttcaggaga tatgcaaagc agaaaccctc gtgcaggccc gaaagagaaa gcgaaccagt
     4801 atcgagaacc gagtgagagg caacctggag aatttgttcc tgcagtgccc gaaacccaca
     4861 ctgcagcaga tcagccacat cgcccagcag cttgggctcg agaaggatgt ggtccgagtg
     4921 tggttctgta accggcgcca gaagggcaag cgatcaagca gcgactatgc acaacgagag
     4981 gattttgagg ctgctgggtc tcctttctca gggggaccag tgtcctttcc tctggcccca
     5041 gggccccatt ttggtacccc aggctatggg agccctcact tcactgcact gtactcctcg
     5101 gtccctttcc ctgaggggga agcctttccc cctgtctccg tcaccactct gggctctccc
     5161 atgcattcaa actgagactc gaggaattcc tgcaggcctc gagggccggc gcgccgcggc
     5221 cgctacgtaa attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     5281 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     5341 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     5401 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     5461 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     5521 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     5581 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     5641 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     5701 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     5761 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtta
     5821 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5881 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5941 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6001 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6061 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6121 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6181 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6241 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6301 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6361 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg
     6421 acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     6481 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     6541 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     6601 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     6661 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6721 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     6781 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     6841 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     6901 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     6961 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     7021 ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat caaaattaat
     7081 ttggtttttt ttcttaagta tttacattaa atggccatag ttg
//
