LOCUS       sequence-137207-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SMARCA2
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(470..490)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(515..532)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             543..565
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    580..604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(604..623)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(605..623)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(707..726)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcattcttct tctttggcaa
      121 ttttctggcg cgcgctttta aacacgctct gcagcacgat gctatcttca taaatctggc
      181 taccttccag gttaaaggtc tgggcattat ggcacagcag catcacatct ttttccagat
      241 cacccaggct acgatattta tggttacgaa tacgttcttt aattttttta aaatccaccg
      301 gtttgcgaat cagttcataa tattccggca gttctttacg gctcggcagc tgaataaaca
      361 cttcgctcag ctggcgaccg ctgctatctt tatagttaat aacggtatca ataatcgcgt
      421 tcatctgttt ggtcagtttc ggcgggttcg ggctcagttt ttccgccatg gattggaagt
      481 acaggttctc ggtacccaga tctacaccag aagaatgatg atgatgatgg tgcatatgta
      541 tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa
      601 ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga
      661 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
      721 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
      781 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
      841 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaa
//
