LOCUS       sequence-137208-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TP53BP1
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(431..448)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(458..475)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             494..516
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    531..555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(555..574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(556..574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(658..677)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgct tagccaagcc
       61 catactgctc tctcagtctg tttccttgct ccaaggacag gatgacagcc attcgcttat
      121 accactttct ttggccttct ttttcaatgc tgtagtacag ttccccagac tccttcctat
      181 gtcctttcac cactcctgca ctgaaatact catcctccga gagggccgtc acttcagtgt
      241 ccagcgggat ggggtcacat aacagaatgt ctttgcccaa cacatcacat tcgtacccat
      301 catcaaagag caatttatac ttcccagctc cgacatctcg tgtgattttc ccagagtaaa
      361 agtagccatt ggatgaccac ttggctacaa cacggagccc tacaaagcta tttcctggag
      421 aggacatatg gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc
      481 tgcccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      541 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc
      601 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      661 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      721 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      781 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      841 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
      901 aaaacctt
//
