LOCUS       sequence-137209-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD10
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(434..454)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(479..496)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             507..529
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    544..568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(568..587)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(569..587)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(671..690)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc aagcttgtcg
       61 acggagctcg aattcggatc cgtatccacc tttactgtca ctcataagta tctttgtttt
      121 gtagggccgc ctggcactct tccaccaggc cttggactag gtagtacttg gcttctgcta
      181 gcagctcctc gatctcccgg cggctctcgg gtaaaggcac cgccccgtct cgaaggtagt
      241 tgagtatcgt accaaagtgc ttcccacagc ggtcaatgag gatccagcct tcactgtcgg
      301 tgagcacttc catgcgcccg cttaacatgg ccttcagcat ggtgtcctgc ttggtcagcg
      361 tctgcatggt tgtatagtag agggctccac ccacattcag cttcacgtat ttggagctgg
      421 ggctcgtgcc catggattgg aagtacaggt tctcggtacc cagatctaca ccagaagaat
      481 gatgatgatg atggtgcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
      541 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
      601 gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
      661 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
      721 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
      781 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      841 ctactggg
//
